The Synthesis Of Transition Metals/Metal Phosphides Nano-material And Its Construction Of Amperometric Biosensor

The transition metal/metal phosphide played an important role in the construction of amperometric biosensor due to its excellent electrochemical performance. In this paper, transition metal/metal phosphide nanomaterials were synthesized, which were used for creating novel platform to immobilize GOx or Hb to fabricate electrochemical biosensor. This work not only enrich the study of biosensors, but also expand application range of the transition metal/metal phosphide nano-materials.1. The silver-copper bimetallic nanoparticles(Ag@Cu NPs) were synthesized via a mild single-step solution method and applied to immobilize glucose oxidase(GOx) and hemoglobin(Hb), respectively. The electrochemical measurements showed that the Ag@Cu NPs significantly enhanced the electrochemical responses of GOx and Hb. Furthermore, the immobilized GOx and Hb remained its good bioactivityand high catalytic activity for glucose and hydrogen peroxide(H2O2), respectively. Meanwhile, amperometric responses showed that the linear ranges were 5 to 3000 μM for glucose and 0.5 to 50 μM and 60 to 135 μM for H2O2, and the detection limits were 3 μM for glucose and 0.3 μM for H2O2, respectively. Compared with other biosensors, the biosensors based on GOx/Ag@Cu NPs/IL/CPE and Hb/Ag@Cu NPs/IL/CPE had wider linear ranges, lower detection limits and higher sensitivities.2. In this work, cobalt phosphide(Co P) on the carbon cloth(CC) as an active matrix was fabricated and utilized as an interface for the construction of Hb-based biosensor. Moreover, the electrochemistry and electrocatalytic properties of Hb on the matrix were investigated. The corresponding results revealed that self-supported Co P nano-material on CC significantly enhanced the electrochemical responses of Hb. In the meantime, the immobilized Hb remained its good bioactivity and showed high catalytic activity toward H2O2. This matrix was a suitable platform to develop high sensitive biosensor, and the linear range of H2O2 was from 2 to 2670 μM with a detection limit of 0.7 μM.3. A novel cobalt phosphide/carbon paper(Co P/CP) was synthetised by the hydrothermal method. The scanning electron microscope(SEM) and electron energy spectrum(EDS) showed that the Co P/CP electrode was successfully prepared. Moreover, the electrochemistry and electrocatalytic properties of Hb on the matrix were investigated. The results revealed that the Co P/CP significantly enhanced the electrochemical responses of Hb. It has been found that the Co P/CP had good electrical conductivity and facilitating electrons transfer between Hb and underlying electrode. At the same time, the immobilized Hb showed catalytic activity toward H2O2, and the linear range of H2O2 was from 37 to 1037 μM with a detection limit of 2 μM.