The Preparation And Properties Characterization Of ADP-ribose Molecularly Imprinted Materials

ADP-ribosylation is a post-translational modification process. It has been found to play a role in all major cellular processes, such as DNA repair, transcription, translation, cell signaling, and death. Furthermore, dysregulation of ADP-ribosylation has been linked to diseases including cancers, diabetes, neurodegenerative disorders, and heart failure, which is a marker in clinical trials. Molecularly imprinted polymer having a specificity and specificity recognition performance to template molecules can simulate the recognition effect of natural biological molecular., MIP is a potential alternative to biological molecules. The paper using ADP-ribosylation of glyceraldehyde- 3-phosphate dehydrogenase(gap A) which has been for reference is aimed at the preparation of a simple, stable and efficient molecularly imprinted materials to identify and gather these protein. According to the method preparation of proteins molecularly imprinted polymers, small molecule AMP is been imprinted instead of imprinting macromolecular protein. Molecularly imprinted nano-gel and core-shell molecularly imprinted polymer were prepared by RAFT method, and identify the ADP-ribose protein successfully. The research content of this paper mainly has the following several aspects:(1) Through the change of nano-gel polymerization conditions change, we found that the factors affecting polymer particle size and Pd I have reaction time, monomer concentration, the content of chain transfer agent and initiator, chain transfer agent content and proportion of functional monomers and cross-linking agent, polymerization temperature. According to the overall trend of the polymer particle size and Pd I, polymer particle size indicates positive correlations with Pd I., controlling the reaction conditions can regulate nano-gel particle size in the range of tens to hundreds of nanometer. A non-target protein BSA, target protein gap A and reference GAPDH were completely adsorpted by MIP and NIP, the adsorption capacity can reach 40μg/mg, there is no difference. This suggests that the protein adsorption quantity gathering by nano-gels itself is very big. The background value of molecularly imprinted nano-gels is too high, leading to no selective recognition effect. We believe that the nano-gel is not suitable for as a protein recognition of molecularly imprinted materials.(2) Polystyrene microspheres were prepared though changing the reaction time. Scanning electron microscopy(SEM) topography and binding experiment were found that the longer the reaction time, the greater size and less uniform the microspheres. The microspheres particle size has great interaction with the adenosine-5’-monophosphate and protein adsorption. When the reaction time is 4h, the microspheres apply to the kernel of core-shell molecularly imprinted polymer.(3) In the preparation of core-shell molecularly imprinted polymer, the longer the reaction time, the better stability and hydrophilic of the polymer in the water. The polymer confusion degree was found to increase from scanning electron microscopy(SEM) topography. When the reaction time is 30 min, the effect of molecularly imprinted polymer specificity and selectivity are the best.The paper has explored the factors influencing the process of nanometer polymer, the regulation of polymerization conditions can meet the demand of different polymer particle size. And by the method of surface imprinting, the core-shell molecularly imprinted materials with specific recognition on ADP- ribose protein are successfully prepared.