The Preparative Process Research And Evaluation On Toxicological And Function Of Ginseng Toad Oil Soft Capsule

Ginseng toad oil soft capsule is a health food which has the enhancement immunity function. It is composed of the ginseng, radix ophiopogonis, toad oil, vitamin E, as raw material, supplemented by beeswax, corn oil research and development. In this paper, the Preparative process were studied, and to evaluate its toxicology and function.After the original medicinal materials inspection,Through orthogonal experiment to optimize the optimum extraction condition :Ginseng is eight times the amount of 70%ethanol concentration for 3 times, extracting time is 1 h; radix ophiopogonis extracted with ten times the amount of water for 3 times, extracting time is1 h. The result of optimized is:determine the sac skin of gelatin and glycerol and water ratio of 2:1:2 sol temperature is80 ℃. Choose corn oil as diluent, and the dosage of 60% of the content, the content of liquidity is good; Choose beeswax as suspension agent, and the dosage of content for 4%,the system has good stability. Ginseng and radix ophiopogonis mixed thick paste vacuum drying time for 36 hours, the water content is less than 5%, crushing grain size is 80 mesh,good stability of suspension system within 12 hours.To explore acute toxicity and mutagenicity of Ginseng toad oil soft capsule by acute toxicity test in mice, bone marrow cell micronucleus test in mice, sperm abnormality test in mice,Amese and 30 days feeding test. Results of acute oral toxicity test in mice is LD50 >21.5 g/kg·BW. ginseng toad oil soft capsule is non-toxic. The result of Amese,bone marrow cell micronucleus tsst, sperm abnormality test are negative.The results of 30 days feeding text are unusual on the observation.The pathological histology observation organs are not seen significant pathological changes. Therefore ginseng toad oil soft capsule is safe and reliable.Function experiment investigates the ginseng toad oil soft capsule to enhance immunity function. Through the mouth give mice with different doses of ginseng toad oil soft capsule for 30 days, can improve the half hemolysis value of mice, antibodies generated cells in mice, which increases the humoral immune function; Can enhance the mice delayed-type allergy, i.e., increase cell immune function; Can improve the mice macrophage carbon clearance ability; For weight gain in mice, viscera/body weight ratio,the transforming ability of mouse spleen lymphocytes induced by ConA, macrophage cell in abdominal cavity of mice’s ability to chicken red blood cells, NK cell activity had no effect. This decision, ginseng toad oil soft capsule has the function of immunity.