Establish And Study On Rapid Propagation Technique System Of Hemerocallis Spp.

In order to be established separately effective rapid propagation systems for4hemerocallismaterials in vitro, in this research, introduced2new varieties of Hemerocallis‘Nameless Pink’and Hemerocallis‘Green Eye’and the hybrid descendant breeds selectively2Hemerocallissuperior individuals RBH and CC627(experimental serial number) was the object of study. Theupside and middle and below of of Hemerocallis pedicel as explants, whose were initiated bycallus induction and adventitious bud induction, thus further subculture on induced adventitiousbud to proliferation cultivation, rooting and smelting transplanting. The experiment summarizesthe correct method on how to build effectively rapid propagation clone in vitro whose4Hemerocallis materials, thus lay the foundation for raising seedlings in industrial scale. Theexperimental results showed that:1. Useing different sterilization program to sterilize as tender degree on upside and middleand below of Hemerocallis pedicel，in order to screen out the best sterilization program. Thecontamination rate and mortality rate reached the lowest when the best sterilization program ofupside pedicel was75%C2H5OH(10s)+0.1%HgCl2(13min); the best sterilization program ofmiddle pedicel was75%C2H5OH(20s)+0.1%HgCl2(14min); the best sterilization program ofbelow pedicel was75%C2H5OH(30s)+0.1%HgCl2(15min).2. Four kinds of Hemerocallis materials presented otherness on initiation medium choice.The optimum initiation medium of WNF and L-1was MS+6-BA1.0mg/L+NAA0.5mg/L+2,4-D1.0mg/L, initiation rate were45.00%and37.60%; the optimum initiation medium ofRBH was MS+6-BA3.0mg/L+NAA1.0mg/L, initiation rate was28.30%; the optimuminitiation medium of CC627was MS+6-BA2.0mg/L+NAA0.5mg/L+2,4-D1.0mg/L, initiationrate was62.53%. The initiation way was indirect adventitious bud for upside pedicel in theinitiation culture, while the initiation way were both indirect and direct adventitious bud formiddle pedicel, but below pedicel was not. Compare with middle pedicel, there was stronger oncallus formation and differentiation capability of upside pedicel, but direct adventitious bud ofmiddle pedicel has a stonger inducibility, it may omit the dedifferentiation process and shortenbreeding cycle, thereforehe the best explant was upside and middle part of pedicel in vitro rapidpropagation of Hemerocallis.3. Four kinds of Hemerocallis materials presented otherness on proliferation mediumchoice. The optimum proliferation medium of WNF and L-1was MS+6-BA2.0mg/L+NAA 0.2mg/L, proliferation coefficient were4.70and4.28; the optimum proliferation medium ofRBH was MS+6-BA3.0mg/L+NAA0.2mg/L, proliferation coefficient was3.28; the optimumproliferation medium of CC627was MS+6-BA1.0mg/L+KT1.0mg/L+NAA0.2mg/L,proliferation coefficient was5.44. It can reach the best proliferation effect when both plantingtype and planting density was designated as three plants for a cluster and every bottleinoculated10clusters in the proliferation culture. The best proliferation time was25d andtransfer times should not be more than7times.4. Four kinds of Hemerocallis materials didn’t present otherness on proliferation mediumchoice. The optimum rooting medium was1/2MS+NAA0.4mg/L+CCC0.5mg/L. If consideringthe cost,1/2MS+NAA0.4mg/L also can be use as the rooting medium., they just existsignificant difference on seeding growth, however,they didn’t exist significant difference onrooting rate and transplant survival rate. Adding to CCC can promote rooting effect and makeseedling stronger, as well as, it also will not affect plant growth after transplantingit. All thetissue culture seedling can survive when seedling grow well (rooting number of every plant wasgreater than or equal to3bar; rooting length was greater than2cm) and was looked afternormally after transplanting.5. Before four kinds of Hemerocallis materials transplanted, hardening-seedling timeshould not exceed2d or directly transplanting as well; the optimum growing media was gardensoil and turfy soil with volume ratio of1:1mixture. If growing media will be treatment throughhigh temperature and high pressure sterilization or tissue culture seedling root will be dipped0.1%KMnO4solution before transplant, it can make transplanting seedlings better growthand enhance transplant survival rate effectively.