| This study used the qinghai plateau unique location advantage and resourceadvantages to carry out jerusalem artichoke germplasm resources ISSR molecularmarkers to research, based on the ISSR data and combining with morphologicalcharacter, analysis of jerusalem artichoke different germplasm resources of geneticdiversity at the molecular level, in order to reveal the differences and fully understandjerusalem artichoke resource, effective conservation and identification of varieties andrich germplasm resources information database, and also provide theoretical basis forfurther explore and character associated marks and carry the carrier, gene localizationand lay the foundation for the variety improvement.The experiment mainly from qinghai plateau jerusalem artichoke plant resourcepool were performance specific24of jerusalem artichoke resource, the2years ofbotany characters survey statistics, and extraction of jerusalem artichoke resource DNAfor ISSR-PCR amplification,combined with botany data and ISSR-PCR amplificationdata jerusalem artichoke resource analysis of genetic diversity.The experimental resultsare as follows:1、To24of jerusalem artichoke resource2years botanical character survey statistics,mainly including the ground part and underground part of the character. The resultsshow that:24of jerusalem artichoke resource tuber character mainly include: fusiform,irregular nodular, rod, ball, wedge, Tubers color mainly has: purple, purple, white,pink, The main characteristics of the part mainly includes: leaf shape, fluff,anthocyanins, etc.2、Through the orthogonal design level of different factors, and screened the bestcombined single factor optimization, the establishment of a jerusalem artichokeISSR-PCR system,the result is:20μl optimum reaction system includes10×PCRbuffer,200μmol/l dNTP,0.5Umol/l primer,1.5mmol/l Mg2+,1.0UTaqDNA polymeraseand50ng template DNA.3、To the jerusalem artichoke ISSR-PCR primers screening, and ultimately selectedamplification banding clear, polymorphism good16ISSR primers.4、Using screen out16ISSR primers to24of jerusalem artichoke resource ISSR- PCR for amplification, the band to the statistics, analysis, the establishment of0-1fingerprint. Results:16primers to24of material were amplification out242clearpath banding, including polymorphism banding article228（91.9%）. The primeramplification banding number in6to22differ, an average of15.125of the primerUBC845get stripe number most, for22, primer UBC844banding the least, for6.Article16the primer amplification polymorphism banding number in3to22differ,an average of14.25. Article16primer amplification of the percentage of polymorphicloci（P,%）range between50%~100%, the average is94.2%. From the amplificationcan be seen in the map, jerusalem artichoke ISSR amplification with grain is rich,amplification of DNA fragment size is in commonly between200to2000bp.5、Combined with24botany traits of jerusalem artichoke resource2years data toISSR molecular genetic diversity analysis, genetic similarity matrix was establishedbased on242bands, the clustering analysis to construct kinship relationship tree,24of jerusalem artichoke resource distribution of genetic distance between0.19~1.01,with an average of0.45, the genetic similarity coefficient was0.68in cutting,24ofjerusalem artichoke resource can be divided into five types: the first class I includeQingyu1; the second class II include W12;III class including: W30, W42, Qingyu2,W09, W18, W26, W51; IV class includes: W06, W84; V class include: Qingyu3,W23, W36, W43, W54, W75, W50, W62, W64, W79, S150, S138, W66, illustratedifferent jerusalem artichoke resource between high genetic diversity. |
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