Effects Of AM Fungi On The Growth Of Scutellaria Baicalensis Georgi Under Different Soil Water And Fertilizer Conditions

Scutellaria baicalensis Georgi is a perennial herbaceous plant from Lamiacea, and itsroot is used for medicine with the function of heat-clearing and damp-drying. It is one of theauthentic medicinal in Hebei province with relieving pain, cooling blood, relievinginflammation and so on. In recent years, with the growing demand, artificial cultivation of S.baicalensis is gradually widely. There are many problems in the process of artificialcultivation: highly decline of quality and productions; and blindly using of water andfertilizer which leads to large, waste widespread water and fertilizer.Arbuscular mycorrhizal fungi are a kind of widespread beneficial microbes which haveclose relationships with soil and host plant in soil. AM fungi can form mutualism withmedicinal plants. AM fungi infect the root of plant and form a huge mycelial network, whichincrease the absorption of water and fertilizer of host plant. It also plays an important role inregulating host plant metabolic activity, promoting the growth of host plants, and increasingplant capacity to stress resistance.In order to creat a better cultivation method using the mycorrhizal biology technology,Pot experiments were conducted to study the effects of different soil water and the infectionof Acaulospora bireticulata on the growth and nutrient content of S.baicalensis. The resultsobtained were as follows:1. Acaulospora bireticulata could infect S.baicalensis effectively. The inoculation ofAcaulospora bireticulata enhanced the AMF colonization rates. Water and fertilizertreatments had significant interactions with the colonization rates of plant roots. When thelow fertilizer conditions with the soil water content of50, the inoculation is best.2. Under different water conditions, inoculation of A.bireticulata promoted the growth ofScutellaria baicalensis, alleviated water stress on the growth inhibitory effects of S.baicalensis, significantly increased the dry weight of S.baicalensis and plant height androot-shoot ratio. The soluble sugar and soluble protein content, POD, CAT, SOD protectiveenzyme activities were higher in the inoculated plant leaves, while the content of proline and MDA was lower. The coloization of A.bireticulata significant improved the content ofbaicalin, P, N, Mg, Ca, Fe, Zn, Cu of plant parts above ground, reduced Mn and Cu contentsof plant root.3. Under different water and fertilizer conditions, inoculate A.bireticulata had differentmycorrhizal effects to S.baicalensis, The inoculation promoted the growth of S.baicalensis,improved plant protective enzyme activity and content of osmotic adjustment substances,decreased proline and MDA contents, these were to contribute to plant drought tolerance. Onthe other hand, the inoculation increased the concent of baicalin, and improved the quality ofS.baicalensis. Water-stress decreased the content of N, P, K, Fe, Cu, while Ca, Mg, Mn, Znincreasing. The content of N, P increase along with fertilizer content improving, othermineral elements get maximum when the fertilizer content M(N0.383g/4kg、P0.564g/4kg、K0.251g/4kg). The inoculation significant increase N, P, K, Ca, Mg, Fe, Zn contents,decreased Mn, Cu contents, improved element nutrition of S.baicalensis.Overall, in the soil water content of50, when the amount of fertilizer applied M, theinoculation effect was best to improve the yield of S.baicalensis and medicinal qualities.Inculationing with AM fungi could improve drought tolerance of S.baicalensis, increase theabsorption of water and fertilizer, so that it result to water and fertilizer saving.