| Helicoverpa armigera is one of the most important pests. Under the appropriateconditions, it can cause large-scale outbreak which causes great economic losses incrop production worldwide. In invertebrate, arginine kinase is one of the key enzymesin energy metabolism. It not only takes part in energy metabolism, but also isinvolved in the ion exchange of the body, immune reaction and resistance to adverseenvironment. The AchE is the target of organophosphorus and carbamate insecticides.Once the decomposition of the neurotransmitter acetylcholine is blocked byorganophosphorus and carbamate insecticides, insects will die of paralysis.The experiment is to study the expression pattern of arginine kinase gene. cDNAsequence of arginine kinase encoding gene was successfully obtained. ExtractedBacillus thuringiensis parasporal crystals were mixed with artificial diet. Thenartificial diet was fed to cotton bollworm. The gene expression level was detected byRT-PCR after treatment. It showed that arginine kinase gene was expressed in eachinstar age, and quantity was different. The gene expression level increased at firstinstar ages to second instar ages, and then decreased, suggesting that the gene isassociated with resistance. The results could provide theoretical basis to research thegene for cotton bollworm.AchE is the molecular target of organophosphate and carbamate pesticide. Onetypes of AChE gene, Ace-2, was obtained from cotton bollworm. The treatmentgroups were fed with the artificial diet mixed with Methomyl. In addition, theRT-qPCR assays were used to detect the gene expression. Each reference gene wasalso used for a2-△△Ctcalculation of the relative expression of gene. The analysis ofgeneral trend indicated that the treatment expression level was higher than controlgroups. The results showed that over-expression of AchE gene could compensate foradapting to pesticide inhibitor. So over-expression of AChE gene could be helpful torapid adaption to new selective-pressures. |
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