Anti-pathogenic Fungal Activity Against Marssonina Brunnea Of Endophytic Actinomyces From Artemisia Capillaris Thunb

Poplar black spot disease is a serious threat to pine forest in China. Nowadays,the research of controlling.Poplar black spot disease by endophytic actinomycetes andactinomycetes have become hot spot in biocontrol research. In this study, a total of349actinomycetes was isolated from medicinal plant Artemisia capillaris, whichcollected from Xuzhou. The cultural media were sodium succinate agar, cellulose-proline agar, trehalose-proline agar, sodium propionate-argintre agar, HV agar andTWYE agar, etc. Each medium was supplemented with K2Cr2O7, rifampicin andnystatin to repressed the gram-negative bacterial and fungal growth.The antifungal pathogenic fungal activities activity of culture filtrate from349actinomycetes strains against Marssonina populi was tested. And the result showedthat75strains had antifungal activity against M. populi. The formentation broth of the75strains which was diluted2times,5times and10times still showedhigh antifungalpathogenic fungal against M. populi.4of the75strains had inhibitingratio of80%against M. populi.The biology characteristics, morphological characteristics, biochemical andphysiological characteristics and16S r RNA sequence of the4strains weredetermined. Based on the reasults, the4strains were identified as Streptomycessp.The16s r RNA sequences hadbeen deposited in GenBank.Then atatistical methods such as Single factor, Plackett–Burman design andresponse surface methodology were employed synthetically to optimize thefermentation media and conditions of the4strainsactinomycetes.And stability test offermentation products of the4strains had been carried out to acid,alkali, temperatureand ultraviolet radiation.