The Quality And Antioxidations Capcity Study On Guizhou Lonicera Fulvotomentosa

【Objects】Lonicera fulvotomentosa in Guizhou, as one of the mainvarieties of Flos Lonicerae Confusae, is an important Caprifoliaceae.Through the research of chlorogenic acid which is the main activesubstance of Lonicera fulvotomentosa in production region in Guizhou,providing theoretical basis for the quality assessment. Meanwhile,seeking production progress of chlorogenic acid, and laying a goodfoundation for the future production, promotion, development, utilizationand industrialization.【Methods】Utilizing HPCL to measure the main active substance ofLonicera fulvotomentosa--chlorogenic acid of different origin andprocessing method in main production areas.Using Acetone/Dipotassiumhydrogen phosphate aqueous two phase extraction to extract chlorogenicacid in primary extract of Lonicera fulvotomentosa, while exploringoptimum process of this method.Quantity of phenolic compounds ofLonicera fulvotomentosa was determined by spectrophotometry, andusing DPPH colorimetric method to study the antioxidant activity ofphenolic compounds, and then taking the advantage of SPSS software toanalyze the correlation between the content and the antioxidant activity ofthe compounds. 【Results】1.HPLC determination showed the content of chlorogenic acidof Lonicera fulvotomentosa in Guizhou is more than2.0%ofPharmacopoeia of People’s Republic of China (2010). The averagecontent of chlorogenic acid was2.67%.2.Comparing with different processing methods, machine dryacquired the highest content of average chlorogenic acid of samples,secondly was steamed dry and air drying was the lowest.3.Chlorogenic acid in primary extract of Lonicerafulvotomentosa could be effectively extracted by usingAcetone/Dipotassium hydrogen phosphate aqueous two phase extraction.The optimum extraction process was that solid-liquid ratio was25:1(mg/mL), acetone-water ratio was8.5:1.5(V/V) and salt-liquid ratiowas100:1(mg/mL). Extraction ratio under this process condition was91.10%, and while the purity of the extract increased from20.16%to42.13%.4.Content of phenolic compounds of Lonicera fulvotomentosa indifferent areas of Guizhou was diverse. The minimum total flavonescontent was10.10%and the maximum was21.60%, the minimum totalchlorogenic acid content was5.60%and the maximum was12.80%, theminimum free radical scavenging rate was24.30%and the maximum was68.50%. 5.Phenolic compounds performed some correlation on DPPH freeradical scavenging rate, moreover total chlorogenic acid made a largercontribution to DPPH free radical scavenging rate and total flavones’contribution was limited.【Conclusions】1.HPLC can effectively determine the content of chlorogenicacid in Lonicera fulvotomentosa, besides the result is stable and reliableand the accuracy is high.2.Content of chlorogenic acid of Lonicera fulvotomentosa inGuizhou is up to the standard of Pharmacopoeia, which shows the highquantity of Lonicera fulvotomentosa in Guizhou and the suitability forplanning.3.Process of machine dry is beneficial to the preservation ofmedicinal components in Lonicera fulvotomentosa and this method issuitable for industrialized production of Lonicera fulvotomentosa.4.Acetone/Dipotassium hydrogen phosphate aqueous two phaseextraction system can effectively improve the purity of chlorogenic acidin primary extract of Lonicera fulvotomentosa and provide a new idealfor chlorogenic acid production process.5.Phenolic compounds of Lonicera fulvotomentosa havepharmaceutical activity of scavenging DPPH free radical, which lead to anew direction for the development and utilization of the phenolic compounds.