| Leukemia inhibitory factor is a member of IL-6family, is a natural multifunctionalcytokine. It was found in M1cells from rat bone marrow leukemia by Tomida in1984, becauseit can inhibit the growth of leukemia cells in bone marrow, so named for the leukemia inhibitoryfactor. LIF gene plays a role of proliferation inhibition, inducing differentiation and promotingapoptosis in some leukemia cells. Leukemia inhibitory factor receptor is widely present in thecell membrane of effector cells. MYCN is a member of Myc family, is a proto-oncogeneexpressed in the embryonic period, N-Myc as the expression product has the activity oftranscription factors, and has the effect that promotes cell proliferation.In order to study the role in regulating the process of tumor diseases of LIF/LIFR andMYCN gene, the experiment with white feather chicken as materials, the avian leukemia virussubgroup J (HPRS-103) inoculated at1day old broiler AA white feather artificially. The relativeexpression of LIF/LIFR and MYCN gene mRNA in eight kinds of tissues before and after virusattacked was detected by semi-quantitative RT-PCR. The results showed that: LIF/LIFR andMYCN gene mRNA in eight types of heart and liver tissues showed a wider distribution, butabundance exist a certain difference. LIF gene expression in the three periods of quantitypresents the first drop after rising trend; LIFR genes expressed in three periods of the amountpresent a downward trend after rising first, and LIFR genes expression quantity weresignificantly higher than that of LIF gene in three times (p<0.05); MYCN gene in three periodsamount of organizations expressed in the experimental group were higher than the control group,and presents the first drop after rising trend.To explore the molecular markers associated with chicken immune traits, the experimentwith Da-gu chicken as test material, PCR-SSCP method was used to detect the geneticpolymorphisms of LIF gene Exon3, LIFR gene Exon20and MYCN gene Exon2, and throughcorrelation analysis in the hope found that single nucleotide mutation associated with chickenimmune traits. The results showed that: a polymorphism locus was not found in Exon3of LIFgene fragment; a transformation of G40569A was detected on Exon20of LIFR gene fragments,the polymorphism loci produced three genotypes AA and BB and AB. For the contents of whiteblood cells, red blood cells, hemoglobin content and erythrocyte deposited four bloodbiochemical index of immune, AB genotype were significantly higher than that of AA and BB (P<0.05), BB genotype was significantly higher than that of AA; BB genotype individual average red blood cell volume was significantly higher than that of AA and AB (P <0.05), no significantdifference between other immune traits (P>0.05). A transformation of C2178T was detected onExon2of MYCN gene fragments; the polymorphism loci produced three genotypes AA and BBand AB. BB genotype individual alkaline phosphatase levels significantly higher than that of AAand AB (P <0.05), and the BB genotype individual content of red blood cell and red blood cellsdeposited were significantly higher than that of AA type (P <0.05), no significant differencebetween other immune traits (P <0.05), The research results provided a reference data for us tofurther confirm LIFR and MYCN gene act as an ant-disease candidate gene. |
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