| The experiment were carried out to study the effects of COS of different levels,as a new green feed addition on egg quality,blood profiles,intestinal morphology,IL-2and TNF-a gene expression in laying hens. A total of480fifty-eighth-weeks old laying hens were randomly allocated to4treatments with4replicates,in each treatments.Each replicate of32hens.The control group was fed basal diet.The test group were fed basal diet that COS was included at300mg,600mg,900mg/Kg in trial diets.The experiment lasted for42days. ADFI, Average egg weight, Laying rate and Feed/egg ratio were calculated each week. Egg Qualities was determined on21d. blood profiles, intestinal morphology,Spleen index,IL-2and TNF-a gene expression were determined on42d.Results:1、Growth performance:The group of300mg/Kg significantly increased the laying rate(P<0.05).While,there was no significant difference in ADFI, Average egg weight,and Feed/egg ratio (P>0.05).2、Egg quality:Compared with the control group,there was no significant difference in Albumen height,Eggshell thickness,Eggshell strength and Egg shape index (P>0.05).Haugh unit of eggs increased as the COS.They respectively increased2.1%,6.87%(P<0.05),6.69%(P<0.05).And,the Yolk/egg ratio reduced with the adjunction of COS,They respectively reduced3.07%,4.43%,5.10%(P<0.05).3、Blood profiles:Dietary supplementation of COS was no significant differently in TP, ALP, GOT, GPT, Ca and P in serum (P>0.05).But the blood sugar reduced significantly.They respectively reduced8.96%,17.4%,21.4%(P<0.05).And,The GOT of600mg/Kg,900mg/Kg was significantly decreased, decreased by10.93%,11.21%(P<0.05).4、Immune and Intestinal morphology.There was no significant differently in Spleen index(P>0.05).Dietary supplementation of900mg/Kg COS have decreased the number of E. coli, and bifidobacteria. The level of the TNF-a Gene Expression in sPleen was significantly higher in600mg/Kg treatment (P<0.05). IL-2gene expression of dietary Supplementation of300mg/Kg,600mg/Kg COS treatment improved. |
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