Transcription Analysis Of Hybrid Offspring Population In Tea Crabapple And Functional Characterization Of MhAN1-like Gene

Apple is very important for the agriculture industry and the farmers’income in China. Rootstock plays a cruicial role in the formation and promotion of fruit yield and quanlity. As well known, apomixis is a desired trait for rootstcok due to its capacity to keep apomictic seedlings uniform in growth. Therefore, it is target for breeders to create apomictic rootstock with other disired traits such as abiotic stress tolerance and strong root system. However, less is known about the molecular mechanism underlying apomictic reproduction, which limits the utilization of apomixis in rootstock breeding. In this study, the transcription profiling of apomixis-related genes were analyzed with high-through approach, and MhANl-like gene was chosen for functional characterization. The main results were telled as follows:1Transcriptome analysis is conducted using Tea crabapple’s hybrid population. At parthenogenesis populations609genes are up-regulated, while723are down-regulated and133genes are up-regulated, while357are down-regulated apomeiosis group. The result showed that the most differentiated genes were involved in biological pathways, molecular function, cell composition, transcription regulator activity, signal transducer activity and defense machanism, as well as physiological and biochemical progresses such as biosynthesis of other secondary metabolites, amino acid metabolism, energy metabolism.2MhANl-like has a short open reading frame (ORF) with582bp, which encoded a21.38kD polypeptide with two ANl domains. IPTG induced MhANl-like expression in prokaryote at37℃.3MhANl protein is subcellularly located in cell nucleus as indicated with MhANl-GFP fusion protein.4The ectopic expression of MhANl-like reduced the tolerance to drought stress in transgenic tomato plants.