| Dairy mastitis is a common disease which is a main reason to result in the economic loss of dairy industry in China. Dairy mastitis is caused by infection of pathogenic microorganisms which led to the inflammation of breast tissue and nipple, it results in the decrease of the milk production and the reduction of milk quality. Antibiotic is the first choice to treat dairy mastitis at present. However, drug-resistant strains and antibiotic residue are in consequence of using antibiotics for long time. So, we should find a more economical, effective, and green treatment to cure dairy mastitis. Mongolian veterinary drugs have many beneficial ingredients which have the function of anti-bacteria, anti-inflammatory, low toxicity, low residual and no side effect. In above researches, Mongolian compound had better effects on treating dairy mastitis. The purposes of this study are to screen the ingredients and compound of anti-bacteria mongolian veterinary drugs and research on their function mechanism. The results are that:1. We selected4ingredients form9anti-bacteria mongolian veterinary drugs by Oxford cup method. They had the anti-bacteria function on escherichia coli (E. coli) and staphylococcus. They had no antagonism effects on the two bacteria and suitable for compatibility after detecting the minimal inhibitory concentration (MIC) and FIC. The MICs of Gallic acid on E. coli and staphylococcus were10mg/mL and1.28mg/mL, respectively. The MICs of Berberine hydrochloride on E. coli and staphylococcus were1.875mg/mL and0.32mg/mL, respectively, The MICs of Rhein on E. coli and staphylococcus were20mg/mL and1.28mg/mL, respectively, The MICs of Rheum emodin on E. coli and staphylococcus were20mg/mL and0.5mg/mL, respectively.2. The two best medicine combinations on the two bacteria were designed according to the orthogonal experiment, four medicines are used on E. Coli. Gallic acid, berberine hydrochloride, rhein are used on staphylococcus with out rheum Emodin. The doses of each medicine were determined by designing the orthogonal experiment, the results are that:the best dose of medicine combination on E. Coli is AlB3CID1, similarly, the best dose of medicine combination on staphylococcus is A3B2C1.3. The MICs of the anti-bacterial medicine compounds are that:the MICs of the first anti-bacterial medicine compound on E. Coli and Staphyloccus were0.78mg/mL. The MICs of the second anti-bacterial medicine compound on E. Coli and Staphyloccus were0.14mg/mL and0.55mg/mL, the MICs of the first anti-bacterial extraction medicine compound on E. Coli and Slaphyloccvs were0.39mg/mL, the MICs of the first anti-bacterial extraction medicine compound on E. Coli and Staphyloccus were0.14mg/mL and0.55mg/mL, espectively. The MICs of ciprofloxacinon on E. Coli and Staphyloccus were0.00125mg/mL and0.0025mg/mL, respectively. The first anti-bacterial extraction medicine compound had the better effect in animal tests, and the protection ratio of mice was62.5%. Although the in vitro anti-bacteria effects of the anti-bacterial medicine compounds were higher than the positive control group, in vivo tests, the protection ratio of the first anti-bacterial extraction medicine compound was similar with ciprofloxacin hydrochloride.4. Through the establishment of mastitis model in mice, the disease was recover after treating with the two anti-bacterial medicine compounds for72h.5. It provided further theoretical basis for optimize the two anti-bacterial medicine compounds through studying the effects of the two compounds on bacterial cell surface characteristics. The effects of the two anti-bacterial medicine compounds on cell membranes of the two bacteria were studied. After the treatments of the two medicine compounds, the cell membrane permeabilities, conductivities, and the contents of alkaline phosphatase (AKP) were increased. Though destroying the cell membrane and increasing permeability, mongolian veterinary medicines played antibacterial effects. |
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