The Effects Of Fermentation Of Two Strains Of Lactobacillus And The Mixed Bacteria On Blood Lipid And Liver Tissue In Experimental Hyperlipidemia Rats

Objective:To observe the effectiveness of two Lactic Acid Bacteria and their mixture on the prevention and treatment of hyperlipemia and fatty liver of the rats.Methods:To establish the hyperlipemia rat model, SD rats were randomly divided into two groups:the control and the experimental group feeding with basic diet and fat-enriched diet respectively. After two weeks, serum total cholesterol and LDL levels of8rats were evaluated. As a result, the experimental group fed a fat-enriched diet were should a significant increase in total cholesterol and LDL levels, and a significant decline in serum HDL which proved hyperlipemia rat model was established. Then, these hyperlipemia rats were randomly divided into six groups:basic diet group, fat-enriched diet group, treated group and three experimental groups.Among thire groups, basic diet group fed with a basic diet, and the others fed a fat-enriched diet. In addition, treated group also were fed with oral lipid-lowering drugs, and three experimental groups were fed with Lactic Acid Bacteria strain1,2and their mixture respectively. Indexes of serum cholesterol and liver function were evaluated every month, and the pathomorphism and histology of liver were inspected simultaneously.Results:the serum total cholesterol levels significantly were reduced in Lactic Acid Bacteria strain1, and significantly increased serum HDL. In contrast, fat-enriched diet group that not fed Lactic Acid Bacteria were observed a severe liver injuried with almost hepatocytes were damaged, interstitial hyperplasia, and the amount of inflammatory cell in hepatic tissue Although observed, liver injuried of treated group and three experimental groups, compared with fat-enriched diet group, was slightly.Conclution:The Lactic Acid Bacteria, particularly strain1, regulated lipometabolism of hyperlipemia rats effectively and could prevent hepatic steatosis, and thus could provide some experimental basis for using Lactic Acid Bacteria in the treatment of hyperlipemia and prevention of Fatty Liver in level.