The Impact Of Fertilizers With Azotobacter On The Eucalyptus Urophylla×Eucalyptus Grandis

In previous work, filter out the nitrogen-fixing Bacillus DL07, based on this experiment, with this bacteria made for fertilizerl, fertilizer2, fertilizer3, fertilizer4and fertilizer5, as basal and top dressing on the Eucalyptus urophylla×Eucalyptus grandis for afforestation.Contrast with eucalyptus fertilizer CK1and self-fixing bacteria with unvaccinated fertilizer CK2,CK3.Determination of test data for afforestation in accordance with fertilizer matrix broth medium, fertilizer bacterial count were divided into four groups are classified. Analysis of nitrogen-fixing bacteria fertilizer on two-years-old trees height,diameter,individual volume,stand volume and chlorophyll content,proline content and nitrate reductase activity of the growth and physiological indexes. The results following:1. The substrate is peat soil, free of inorganic nitrogen, nitrogen-fixing bacteria of different amounts of fertilizer1(3×109-4×109个/g), fertilizer2(4×109-5×109个/g), fertilizer3(9×109-10×109个/g) of CK1(eucalyptus special fertilizer) and CK2(excluding fixing bacteria) comparison of the results, tree height is CK2> fertilizer1> fertilizer3> fertilizer2> CK1, DBH is fertilizer1> Fertilizer2> CK1>CK2> fertilizer3, the average individual volume and forest stand volume is fertilizer1> CK2> Fertilizer2> CK1> fertilizer3, manure chlorophyll content is fertilizer1> CK1> fertilizer2> fertilizer3> CK2, proline is fertilizer3> fertilizer2> Fertilizer1> CK1> CK2, nitrate reductase activity is the fertilizer1> CK1>fertilizer3> Fertilizer2> CK2. In addition to nitrate reductase activity, the rest of index analysis of variance among treatments were significant differences did not reach the level. Description in peat soil as a substrate of inorganic nitrogen tertilizer with nitrogen-fixing bacteria were the same fertilizer.2. The matrix is applied cassava residue, fertilizer bacterial content of the9×109-10×109个/g, compared with free of inorganic nitrogen fertilizer4and self-made compound fertilizer CK3and CK1, eucalyptus trees height, diameter at breast height, trees volume and stand volume lower than the CK3and CK1, chlorophyll content is CK3> fertilizer4> CK1, proline is the fertilizer4> CK3> CK1, nitrate reductase activity is CK3> fertilizer4> CK1. In addition to chlorophyll content, the remaining indicators analysis of variance among treatments were did not reach the level of the significant differences. Instructions for composting cassava residue as a substrate with free of inorganic nitrogen fertilizer were similar with the contrast.3. Fertilizer substrate is peat soil, the same amount of bacteria on the fertilizer3and the fertilizer5, culture medium were Azotobacter beef extract peptone and Ashby medium were compared. DBH, tree average individual volume and forest stand volume are fertilizer3> fertilizer5, and tree height growth are fertilizer5> fertilizer3. Promotion effect on tree growth, the beef extract peptone culture was superior to Ashby fertilizer formulated medium. Chlorophyll is fertilizer3lower than fertilizer5, proline is fertilizer3than fertilizer5, two kinds of fertilizer nitrate reductase activity was essentially flat. ANOVA results of the indicators were significant differences did not reach the level of proof used as fertilizer in the two kinds of nitrogen-fixing bacteria DL07culture medium had no significant effect on fertilizer.4. The fertilizer3and fertilizer3are manure compost peat and cassava residue as fertilizer formulated matrix. Comparison of the two results:average diameter, forest volume and stand volume are the fertilizer3more than fertilizer4, the average tree height fertilizer4> fertilizer3. On tree growth promoting effect, with peat as a substrate fertilizer better than compost cassava residue matrix. The fertilizer3content of chlorophyll and proline content were heighter than the fertilizer4, and nitrate reductase activity is fertilizer4heighter than fertilizer3. ANOVA results, two individual volume of timber handling and stand volume per unit area was significantly different, the rest of the indicators ANOVA results did not reach significant difference. It proved to be used as fertilizer peat soil matrix better.5. This experiment prepared five kinds of fertilizer for the trees growing role: tree height growth aspect is fertilizer5> fertilizer1> fertilizer4> fertilizer3> fertilizer2; while DBH is fertilizer1> fertilizer2> fertilizer3> fertilizer4> fertilizer5; forest tree volume and stand volume are fertilizer1> fertilizer2> fertilizer3> fertilizer5> fertilizer4. Comprehensive comparison to the fertilizerl on forest growth promoting effect best.