Research On Protection Of Aloe’s Anthraquinones On Growth And Development Of The Soybean Under UV-B Radiation Stress

In recent years, with The rapid development of industry and agriculture on the global> activitives of human also made a large of damage to the nature, the fluorine and chlorine hydrocarbon compounds emissions serious damage of atmospheric ozone layer, lead to earth’s ozone layer thinning even areas appear empty, so the sun’s ultraviolet radiation that reaches the earth’s surface has increased。At present about enhanced ultraviolet radiation damage to the plant has a large number of research reports, but how to defense or mitigate enhanced ultraviolet radiation damage to the plant research data is less。In this paper, application methods of plant anatomy, plant chemistry and plant physiology under the condition of artificially enhanced ultraviolet radiation, to study the influence on soybean’s leaves morphological structure and physiological and biochemical indexes under Spraying of anthraquinone in aloe。In order to Explore enhanced ultraviolet damage to soybean and the protection of anthraquinone in aloe on soybean under UV-B stress protection, get the following results:1. Under the condition of artificially enhanced UV-B radiation, plant height, leaf area of soybean plant without spraying anthraquinone in aloe decreased obviously, lower than CK group by23.43%,23.43%respectively, blade thickness increased21.40%than CK group; In the same conditions, plant height, leaf area of soybean plant which spraying anthraquinone in aloe descend range was small, T2group falling range was4.60%、5.71%, were the lowest, and this group of the blade thickness increased by2.58%, was very close to the level of CK group.2. Under enhanced UV-B radiation and without spraying anthraquinone in aloe, content of photosynthetic pigments, leaf stomatal conductance, net photosynthetic rate, DW/FW, biomass, all of which had fallen dramatically; but the relative electrical conductivity, malondialdehyde, POD activity and SOD activity generally increased; When spraying anthraquinone by the concentration of0.5mg/L, the total amount of chlorophyll, leaf stomatal conductance, net photosynthetic rate, DW/FW, biomass, which are lower than that of the CK. group by4.12%.8.82%.6.29%.4.55%.3.03%; the relative conductance rate, malondialdehyde, POD activity and SOD activity were higher than that of the CK group by5.23%.10.81%.8.24%.13.04%.3. From the photos of scanning electron microscope, epidermal cells of soybean leaf were clear and regular and arrange orderly. Under natural light, stoma subsidence had no obvious subsidence phenomenon; but in the ultraviolet light, waxy substance on the surface of leaf cells significantly increased, the stomas subsidence was clear, local cellular structure was destroyed; after spraying aloe anthraquinones, waxy substance on cell surface also increased, but it was not much, and the situation of stoma subsidence ease obviously, whole cells structure and shape were similar to that of natural light.All kinds of morphological structure and physiological and biochemical indicators prove that enhanced ultraviolet radiation can have made different degrees of damage on soybean leaf structure and all physiological。Spraying a certain concentration of aloe anthraquinones on the surface of soybean can effectively reduce the damage of UV-B radiation, because aloe anthraquinones can absorb and consume UV radiation energy for protecting plants against the damage of ultraviolet radiation. Therefore, spraying a suitable concentration of aloe anthraquinones on the surface of plants was a new effective way to prevent the plants form the damage of UV radiation.