TvNHX1Gene Transform Petunia Hybrida By Agrobacterium-mediated And Responses Under NaCl Stress

Soil Salinization is one of the major problems that the environment faced,Salinization of the soil has a lot of salt and harm ions, this harmful ions’ accumulationwill affect the normal growth of plants. Therefore, improving the land environment andimproving urban green plants’ salt tolerance has important practical significance.Petunia hybrid, is ornamental grasses, are commonly used as a landscaping urbangreening plants. In this thesis, TvNHX1convert into Petunia, Hope to increase thepetunia tolerance in saline environment, Provide resources of plants growth on salineland. This trial transfer TvNHX1into petunia by Agro bacterium-mediated genetictransformation methods, And carried out a preliminary analysis of salt response, themain content and experimental results of the study are as follows:By Agro bacterium-mediated genetic transformation method, P3300-TvNHX1vector transfer into petunia plants, after PPT screening, obtains resistant plants fortarget gene identify by PCR, received24lines T0generation petunia positive plants.Using tissue culture rapid asexual propagation techniques, obtained individual petunia.These petunias treatments with two stress mode, analysis with the extension of theNaCl stress time and increase the concentration of the NaCl, transferred TvNHX1andnon-transgenic petunias differences in physiology and gene expression.Through analysis and comparison of transgenic and non-transgenic petunia whensubjected to salt stress soluble proteins, changes in SOD、POD、 CAT activities，andMDA、Na+、K+、Ca2+、Mg2+content six physiological indicators can be found, whena low level of salt stress suffered petunia plants, Petunia plants showed that PODsynergistic SOD format self-defense system to resist the salt stress against, when ahigh level of salt stress suffered petunia plants, Petunia plants showed that CATsynergistic SOD format self-defense system to resist the salt stress against. Buttransgenic plants vivo SOD, POD, CAT activity in general were higher thannon-transgenic petunia. Meanwhile, with the salt stress following, MDA content inpetunia plants will increase, But the MDA content in transgenic plants is still less thanthe non-transgenic plants, Illustrated by three synergistic protective enzyme reduces the per oxidation of the plasma membrane. Under NaCl stress conditions, transgenicpetunia Na+content did not increase, close to under non-stress conditions Na+levels;K+levels did not decrease, maintain a steady state; Ca2+content has a growing trend;Mg2+content remained unchanged, suggesting TvNHX1gene may play a positive rolein maintaining ion balance.RT-PCR analysis the non-transgenic petunia and transferred TvNHX1petuniawhen subjected to salt stress gene expression levels of SOD, SOD gene expression intransected petunia was higher than the non-transgenic petunia under NaCl stress.Following a higher NaCl concentration or a longer time of NaCl stresses, TvNHX1intransgenic petunia expression appear upward trend.