| In the present work, a novel high affinity adsorbent for deoxynivalenol (FH-FCC, main components are calcium base montmorillonite and carbonization-montmorillonite) was constructed. The objective of this study was to evaluate the ability of FH-FCC to adsorb deoxynivalenol (DON) in vitro and the efficacy of FH-FCC to protect against mycotoxicoses of piglets.In experiment one, twelve adsorbents were tested adsorption of DON in vitro and the results showed that adsorbent D, adsorbent FH-FCC and granular activated carbon (GAC) had high binding capacity of DON (the adsorption rate of DON were100%,94.90%,40.43%, respectively). Based on this study, DON binding capacity by these three adsorbents at pH2.0,6.0, simulated gastric fluid condition was compared. The results indicated that:(1) Adsorbent D showed the highest DON-binding ability, followed by adsorbent FH-FCC and GAC.(2) The desorption rate of DON from adsorbent D, adsorbent FH-FCC and GAC was low. Therefore, adsorbent D and adsorbent FH-FCC showed high binding ability for DON in vitro condition.In experiment two, the effect of adsorbent FH-FCC on the protection of IPEC-J2cell against cytotoxicity induced by DON were tested. Results showed that at levels of0.2,0.5,1,2μg/mL of DON and duration of exposure of24,48h, relative survival index of IPEC-J2were greatly decreased. Dose-response relationship was observed when DON concentration was low and exposure duration was short. Addition of FH-FCC to different concentration of DON and duration of exposure of24,48h showed that,(1) after24h’ exposure, adding FH-FCC to different concentration of DON help significantly increase the relative survival index of IPEC-J2cells.(2) after48h’ exposure, adding FH-FCC to0.2μg/mL of DON help significantly increase the relative survival index of IPEC-J2cells.In experiment three, in vivo efficacy of adsorbents as DON binders in piglets exposed to DON-contaminated feed was conducted. Two hundred weaning piglets at (36±4) days were randomly allocated to5dietary treatments with4replicates per treatment and10piglets per replicate. Piglets in NC group were fed a basal diet (normal maize, negative group); piglets in FH group were fed a basal diet with FH-FCC; piglets in DON group were fed the diet with deoxynivalenol-contaminated maize (normal maize in the NC group was100%replaced by deoxynivalenol-contaminated maize, positive group); piglets in DON-FH group and DON-D were fed the diet with deoxynivalenol-contaminated maize respectively supplied with2%o FH-FCC or adsorbent D. The trial lasted for28days. After28days’ trial, every six piglets from NC group, DON group and DON-FH group were chosen and slaughtered. Results showed as below.(1) In the first two weeks, compared with NC group, average daily gain (ADG) were significantly reduced and feed/gain (F/G) significantly increased in DON group; adding FH-FCC to DON-contaminated diet help recover the DON-decreased performance; adding adsorbent D cannot help recover the DON-decreased performance.(2) No significant difference in pig growth performance and other related serum parameters were found between NC group and NC-FH group.(3) The DON-contaminated diet can significantly reduce antioxidant ability and immune competence, do harm to intestinal structure of piglets. Adding FH-FCC to DON-contaminated diet help eliminate these adverse effects to piglets.In conclusion, after in vitro tests, cytotoxicity tests of IPEC-J2cell and in vivo tests, adsorbent FH-FCC could bind DON effectively. These results indicated that FH-FCC could alleviate some of the toxic effect in piglets. It might prove to be beneficial in the management of DON-contaminated feedstuff’s for pigs. |
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