| Three experiments were conducted to determine the adsorption characteristics and effects of mycotoxin adsorbents on aflatoxin B1 (AFB1) in Feed. The property of adsorption was evaluated, utilizing adsorption isotherm to selecte suitable adsorbents, and further tests were taken on the vitro sorption and vivo efficacy of AFB1Exp.1, The Study of Characterics of Absorbents on AFB1Vitro sorption of AFB1 on five adsorbents were characterized, two commercial adsorbents and three contrivable adsorbents were selected to sorb aflatoxin B1 (AFB1) as below:adsorbent A, main component is yeast cell extracts; adsorbent B, main component is HSCAS; adsorbent C, the compound of yeast cell extracts and HSCAS; adsorbent D, the compound of activated carbon and HSCAS; adsorbent E, the compound of activated carbon and yeast cell extracts. Adsorption isotherms were used in this test to describe the adsorption characteristics. Results showed that adsorbents fitted to the Freundlich isotherm, and suggested a multilayer of molecular of AFB1 adsorbed on adsorbents for their adsorption. For their greater adsorption quantity we chose adsorbent B and adsorbent D for further study.Exp.2, The Vitro Study of The Capacity of Absorbents on AFB1The adsorption capacity of the chose adsorbents was investigated by the follow condition:(1) AFB1 binding capacity by two adsorbents at pH 2.0,5.0,6.0,8.0, was compared, (2) AFB1 binding speed by two adsorbents in vitro condition was compared, (3)The stability of adsorbent-AFB1 complexes in vitro condition was conducted, (4) The binding capacity by two adsorbents to three degree of contaminated maize were compared. And primary results indicated that:(1) Adsorbent D showed the higher in vitro affinity for AFB1, than adsorbent B, (2) Adsorbent B bound 97.69% of the AFB1 in solution in 90min, and 96.03% adsorption rate by Adsorbent D, (3) Adsorbent B-AFB1 complex was much stronger than Adsorbent D-AFB1 the in vitro condition, (4) Both adsorbents showed high binding capacity for AFB1 in three extent contamination maize. It implied that:two adsorbents had a great detoxification capacity, strong adsorption adhesion, and short binding time, could form stable Adsorbent-AFB1 complex. Additionally, adsorbent D showed a greater capabilit than adsorbent B.Exp.3, Effect of Absorbents on Producing Performance of Growing Pig In vivo efficacy of two adsorbents as aflatoxin binders in finishing pig exposed to aflatoxin-contaminated feed was assessed. A total of 20 pigs were randomly allotted to four treatments (one pens per treatment of pig). As a result, feed intake (FI) and average daily gain (ADG) decreased (P>0.05) and feed gain ratio (F:G) increased (P>0.05) in the treatment fed aflatoxin-contaminated feed versus the control treatment. The data suggested that the two adsorbents tested alleviated the adverse effects on the growth preformence of finishing pigs caused by aflatoxin, while compared with adsorbent B, adsorbent D could alleviate the toxic effect of AFB1 more effectively.In conclusion, adsorbent D could bind AFB1 effectively both in vitro and in vivo. The adsorption is chemical interaction. These results indicated that adsorbent D could alleviate some of the toxic effect in swines. It might prove to be beneficicial in the management of aflatoxin-contaminated feedstuffs for swine. |
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