| Melon (Cucumis melo L.), is a very important horticultural crop worldwide which belonging to Cucumis, Cucurbitaceae. Furthermore, melon is also one important economic crop worldwide which plays a significant role in farmers’ income through melon produce. With the deepening of reform and opening up, the state of melon production quickly increases,the melon industry’s rapid development.At present, the phenomenon of homonym and synonyms in melon industry of our China are widespread, which could cause variety authenticity issues. Also, because of the unstrictness in breeding, processing, storage and other aspects, there are many seed quality problems, such as poor varietal purity. There are many reasons for these problems, and now the effects of these problems in melon production are tremendous. In order to solve the disputes and confusions in the market during the course of melon seed operating, it’s necessary to establish DNA fingerprint database of melon, which could provide technical supports for the identifications of varietal purity and authenticity. At the same time, as the second largest originating center of melon in the world, China owns rich melon material resources, so it’s important to enhance the basis study of melon and establish an information platform about the public melon germplasm genetic relationship. In this study,235melon from China melon market were employed to reveal their genetic diversity and morphological characters based on SSR markers. And The results were demonstrated as follows:In this study, the genetic diversity in235melon aceessions was assessed using14morphological descriptors. The morphological deseritors include7qualitative and7quantitative. The average coefficient variation was0.17. The morphological traits revealed the variance coefficent on melon aroma reached0.46. They ranged from0.02in covered veins on fruit rind and the color of covered veins to0.46based on7qualitative descriptors and from0.05in number of days after sowing to harvesting to0.24in weight of fruit based on the7quantitative descriptors.The result of morphological cluster analysis showed that the genetic similarity coefficient of the235accessions ranged from0.73-0.96.60materials were extracted for the second cluster analysis, and the similarity coefficients were between0.73-0.94, also, the test materials could be divided into2categories at the genetic similarity coefficient of0.74. The results indicated that based on morphological cluster analysis these melons could be classified into Cucumis melo ssp. conomon and C. melo ssp. melo.The genetie diversity in melon accessions by SSR.The results showed:SSR markers by18core primers were used to determine the frequence of DNA Polymorphism in melon germplasms. A total of157bands were detected among235melon accessions. Each pair of primers could detect4-14polymorphic bands, and the average were9bands. Also, the average of polymorphism information contents was0.71with the range of0.57-0.87. Based on157sites of18pairs of core primers, we obtained the cluster analysis pictures of235materials, with the similarity coefficients between0.69-0.99.60materials were extracted for the second cluster analysis, and the similarity coefficients were between0.75-0.95, also, the test materials could be divided into6categories at the genetic similarity coefficient of0.77.Based on the amplification of18pairs of primers, fingerprint codes for each material in235melon varieties were established, and there were10groups of materials with the same fingerprint, involving26materials that accounted for12%of the tested materials, and the identification rate reached88%.And the other accession had unique fingerprinting.Also, fingerprint QR codes of each material were constructed, including name, origin, color, type, quality, SSR fingerprint and... |
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