Research On Enzymatic Properties Of Antioxidase From Pholiota Namekio And Cell Fusion In Similar Species

In recent years, people gradually began to focus the antioxidant effect ofmushroom. Active substance and content contained different types of mushroom, therole will be different. The research to the antioxidant of Pholiota namekio, helps tomake full use of its active ingredient. This study optimize the liquid fermentationconditions of Pholiota namekio by univariate methods. Research the enzymaticproperties of antioxidant enzymes of mycelium, deeply understand the capability andquality of antioxidant enzymes of mycelium. It provide a theoretical basis for thedevelopment of new functional foods. By the experiments of cell fusion,it can establishnew strains and screen the fusants with high antioxidant capacity,lay the foundation forbreeding of excellent srain of Pholiota namekio.Through the measuring and the fuzzy comprehensive evaluation to the biomass ofmycelia, content of protein and polysaccharide and the fuzzy comprehensive evaluation,it can screen advantage strains of Pholiota namekio. The results showed that the degreeof strain outstanding: Pholiota namekio1> Pholiota namekio C3-1> Pholiota namekioRH> Pholiota namekio5188> Gong-yu3.Test change liquid culture conditions of Pholiota namekio to improve biomass andantioxidant capacity of mycelium. Study the effects of temperature, pH, salts on them.obtain of liquid fermentation conditions after optimization: potato20%, glucose2%,peptone0.5%, VB10.001%. The formula of the adding of salts: KH2PO40.1%, MgSO40.1%, CaCl20.01%, the initial pH value:7to7.5,20℃ambient dark after inoculation.The test extract GPx, PPO, CAT, SOD from the cultured mycelia of Pholiotanamekio, the enzymatic properties of four antioxidant enzymes were studied, the foundafter testing: Pholiota namekio Gpx activity reached a maximum at30℃, its activityinfluenced by temperature, and have bad thermal stability, the optimum pH was6.0, Na+promote the performance of GPx vitality; the optimum temperature of PPO at about40℃, and have good thermal stability, the best fit pH is6.0, the adding of Na+and Mg2+make performance of PPO enzyme activity improve; the optimum temperature ofPholiota namekio CAT at about40℃, the difference of CAT activity is not obvious atpH5.0-7.0, have reached a higher level, Na+promote the CAT activity performance; the optimum temperature of Pholiota namekio SOD at about40℃, it still maintainhigh activity when the temperature reaches90℃, the optimum pH was5.0, Mn2+play acatalytic role on SOD.Tests showed that, the fusion that make Pholiota namekio1, Pholiota namekio C3-1and Pholiota namekio RH as parents,13fusion strains were obtained.Six of them areregression. The passaged capability of R4, R14and the clearing capability of O2-aresuperior to other strains.