Studies On Culture And Polysaccharide Of Pholiota Adiposa (Fr.) Quél.

By using Pholiota adiposa (Fr.) Quel. as the experimental material, which is isolated from woodland, this research is mainly about solid and liquor medium, and the extraction technology of polysaccharide of Pholiota adiposa. By the experiments of Removing Free Radical, Blood Serum Hemolysin (Body fluid Immunity Function), and Phagocytosing Ratio and Phagocytosing Exponent of Macrophage (Monocaryon-Macrophage Function), the bioactive of A1 (polysaccharide extracted from fruit body of Pholiota adiposa (Fr.) Quel.), A2 (polysaccharide extracted from mycelia of Pholiota adiposa (Fr.) Quel.) and PA (pured polysaccharide extracted from fruit body) are tested. The results are as follows:1. By comparing the influence of different solid medium ingredients (GYM, synthesis PDA+apple juice, Yangshi, synthesis PDA and MMN) on the growth of mycelia of Pholiota adiposa (Fr.) Quel., it is showed that the relatively ideal culture medium under experimental condition is synthesis PDA culture medium.2. According to the method of single factor analysis and orthogonal design, it is concluded that the best formula of liquor medium is: Glucose 2%, Yeast extract 0.8%, V_B1 300 μ g, Mg₂SO₄ 0.04%, KH₂PO4 0.1%, K₂HPO₄ 0.1%.3. Studies reveal that the best liquor cultivative condition of Pholiota adiposa (Fr.) Quel. is: 3 inoculation pieces, under 25℃, pH 5.5, rotating speed 120r/min¹40r/min for 12 days.4. After the studys on the cultivation technology of Pholiota adiposa (Fr.) Quel., a suit of high yield cultivation technology is achieved.5. After the experiments of single factor analysis and orthogonal design, the best extracting technics of polysaccharide in mycelium of Pholiota adiposa (Fr.) Quel. is fixed, that is, grinding diameter 160 order, volume of water(mutiple ) 1:25, time of extraction 2.5h> temperature of extraction 80 ℃ , one extraction, when it comes to the deposition of polysaccharide from extraction liquor, the best way is to add in absolute ethanol, 3.5 times of the extraction liquor and separate for 12 hours.6. The experiments of Sevag method and ion exchanging method are carried out to remove the protein form coarse polysaccharide. The result shows that, there is a relatively good effect when disposing the coarse polysaccharide using Sevag method two times, the protein removing ratio is 70% and the recycle ratio of polysaccharide is 66.5%, but ion exchanging method doesn't have a good result, the protein removing ratio is only 31%.7. The result of the experiment of paper chromatography shows that the polysaccharide extracted from mycelia of Pholiota adiposa (Fr.) Quel. is glucan. But the paper chromatography method isn't precise to determine the component of this polysaccharide, so further experiment is expected to fix on its component.8. The experimental results of Removing free radical show that A1 (polysaccharide extracted from fruit body of Pholiota adiposa (Fr.) Quel.), A2 (polysaccharide extracted from mycelia of Pholiota adiposa (Fr.) Quel.) and PA (pured polysaccharide extracted from fruit body) all have a good effect forremoving free radical.9. Al (polysaccharide extracted from fruit body of Pholiota adiposa (Fr.) Quel.) and A2 (polysaccharide extracted from mycelia of Pholiota adiposa (Fr.) Quel.) are positive in the experiment of Body Fluid Immunity Function;A2 (polysaccharide extracted from mycelia of Pholiota adiposa (Fr.) Quel.) and PA (pured polysaccharide extracted from fruit body) are positive in the experiment of Monocaryon-Macrophage. According to the determination principel of "Technical Standards for Testing and Assessment of Health Food", when both of the two experimental results are positive we can say the sample can enhanc Immune Function. So A2 (extracted polysaccharide from mycelia of Pholiota adiposa (Fr.) Quel.) has the immune enhancement.