MRNA Expression Pattern Of Three Monosaccharide Transporters In Small Intestine Of Broiler And Layer

In chicken small intestine, SGLT1and GLUT2are two important glucose transporters, and GLUT5is mainly transporter for fructose. They are closely related to the monosaccharide absorption. In order to study their effects on growth traits, the body weight, the weight of small intestine, the length of small intestine of Aviagen and Leghorn chicken were measured and the mRNA expression of SGLT1, GLUT2and GLUT5were determined using real time PCR at different developmental stage. The data were obtained and analyzed by using SAS software. The results indicated that:(1) The body weight, the weight of small intestine and the length of small intestine of broiler were higher than that of layer significantly (P<0.01).(2) The difference of the mRNA expression of SGLT1, GLUT2and GLUT5at different developmental stage were highly markedly (P<0.01), the quantity of SGLT1and GLUT2expression seem to be influenced by breeds (P=0.09, P=0.08).(3) There was negative correlation between the growth traits and the expression of SGLT1, GLUT2and GLUTS. From day1to day7, the expression of SGLT1, GLUT2and GLUT5changed greatly. After highest expressed on day1or3, their expression declined down and tended to stable.(4) The expression pattern was same for layer with different diets but that was different for broilers. The influence of diets for broilers was bigger than layer. The expression pattern of broilers were different mainly in the early days (before day7), and that was same after day7.(5) When fed with the compatible feeds, the expression pattern of SGLT1and GLUT2were same in broilers and layer, but that were different for GLUT5.In conclusion, the study laid the foundation of breeding for improving feed conversion rate.