Infectivity And Community Structure Of Arbuscular Mycorrhizal Fungi In The Vegetable Greenhouse And Their Effects On The Tomato Growth

Overuse of fertilizer and in appropriate fertilizer application mode in the vegetable-cropping system of China caused the amount of soil beneficial microorganism decreased greatly, and become a main restricting factor for agriculture sustainable development. Arbuscular mycorrhizal fungi (AMF) are soil fungi which form a mutualistic symbiosis with the roots of most crop plants. An understanding of the impacts of fertilization practices upon communities of these fungi would help to ensure an opportunity for the utilization of the symbiosis and contribute to the success of sustainable agriculture.In this lecture, we selected Shouguang Field Experiment of China Agricultural University as our experimental site, study the influence of different amount of chemical nitrogen and the organic manure input on soil physical and chemical properties change, the modifed mean mycorrhizal infection percentage (MIP), and AM fung community at different soil layers (0-30,30-60cm). In addition, the effect of AM fungi on tomato was studied that grown in the continuous cropping soil. The results were shown as below.MIP was significantly restrained by fertilization, but not differed among the nutrient input modes and dose.Terminal restriction fragment length polymorphism (T-RFLP) and clone-sequencing methods were employed in this study to comparative investigate AMF community response to different nutrient input mode and dosage. Six phylotypes of AM fungi in Glomeraceae, Diversisporaeae, Acaulosporaceae was fond in in0-60cm soil layer of Shouguang fiels experiment. Among them, Funneliformis mosseae and Rhizophagus intraradices was very common found in the crop field. Compared AM fungal community at different fertilization treatments in0-60cm soil layer, AMF community composition and structure ware found have significant difference between0-30cm and30-60cm soil layer.Analysis the relationship between soil conditions and AM fungal community, we found soil pH, organic matter, available phosphorus and total nitrogen were significantly correlated with AM fungal community composition and structure. But the correlated factor varied in different soil layer. In addition, species richness was affected by soil pH, EC and organic matter.In the pot experiment, AM fungi inoculation significantly increased tomato fruit biomass.