| The aim of this study was to explore the feeding behavior of Sitobion avenae underlong-term exposure of Zn2+.In the simulative natural environment, the wheat in the soil wasirrigated with different Zn2+solutions. S. avenae was treated for20generations consecutivelythrough soil-wheat-aphids system. The1st,5th,10th，15thand20thgenerations of adult aphidswere collected and analyzed using EPG.The results showed that,1. Even though the total duration of np and C were decreased at200mg/kg of zinc, theywere increased at20thgeneration. The duration of np waveform was longer at the20thgeneration than the1stgeneration. Similar resulst were obtained for number of C withduration of np.2. Durations of G were prolonged with different concentration of zinc. The high dose ofZn2+increased the difficulty on phloem ingestion from the xylem significantly at the15thgeneration.3. About the research on penetrating phloem of aphids,no significant influence was foundabout the duration of E1involved in salivation and number of E2waveform involved inpassive feeding in low-dose of Zn2+. However, the durations of single E1, E1followed bysustained E2and numbers of E2were decreased by high-dose of zinc. However, the durationsof single E1, E1followed by sustained E2and numbers of E2were decreased by high-doseheavy metal.4. The total duration and number of np and C were lowere at200mg/kg of zinc,whilethey were higher at the high-dose of zinc.It was conclude the feeding process is effectivelyreduced.5. There was no F waveform during20generations in different concentrations of zinc.The conclution were,1. There was accumulation effect.2. Getting water from xylem was influenced on Sitobio avenae by zinc.3. Phloem ingestion was difficult.4. The feeding behavior could be induced by low dose of Zn2+, while inhibited byhigh-dose.5. The feeding might be not hindered in cell walls and gaps between cells. |
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