The Drug Screening Of Chinese Medicine Against Mycoplasma Suis In Vitro

In recent years, Mycoplasma suis spread extensively all over the country with the rapid development of swine industry, this not only made swine grow and develop slowly, slaughter lately, reduce production performance, but also led to a huge number of swine’ death. After infected, swine always were recessive, and this often caused other secondary infection or mixed infection. This made it difficulty to diagnosis and prevention, caused greatly hinder to the sustainable development of swine industry, so it should be attached great importance.The paper adopted the method of culture in vitro, chose8traditional Chinese medicines have the function against bacteria and virus, they were radix isatidis, Artemisia apiacea, coptis chinensis, radix bupleuri, lonicera japonica, fructus forsythia, Houttuynia cordata thumb, Baical Skullcap Root. Culture medium added traditional Chinese medicines with different concentration was put into the incubator, and then culture Mycoplasma suis under the condition of37℃and5%CO2, at the same time set up the control group without medicines for comparison, watching the situation of Mycoplasma suis attached to red cells at different time periods, working out their infection rates, extracting DNA of Mycoplasma suis for RT-PCR to analyse the result of Chinese medicine against Mycoplasma suis.The results showed that Artemisia apiacea and Baical Skullcap Root at4000μg/mL and2000μg/mL obviously inhibited Mycoplasma suis to attached to red cells, followed by coptis chinensis at4000μg/mL and2000μg/mL, however, radix isatidis, radix bupleuri, lonicera japonica, fructus forsythia, Houttuynia cordata thumb and the control group without any medicine had no obvious difference.The results proved that Artemisia apiacea and Baical Skullcap Root obviously inhibited Mycoplasma suis to attached to red cells, the next was coptis chinensis, but other medicine had no obvious difference.