Effect Of α-lipoic Acid,Vitamin P And L-carnitine On Boar Sperm Preserved At17℃and4℃

Artificial insemination(AI) in the pig production industry make the widespread ofgenetic material of superior boar possible, leading to huge improvement in pig breeding. AIalso makes the preservation of boar sperm more and more important.At present, cryopreservation and liquid preservation are two ways used for the storage ofboar sperm. Due to highly sensitive to temperature change of boar sperm, cryopreservation ishardly used for boar sperm preservation for AI. Liquid preservation of boar sperm is still themain way for AI in pig production.Oxidative stress damage of boar sperm happens when processing of boar sperm lead toexcessive free radical which will result in damage on sperm membrane, protein and DNA. Inthis study, we aim at investigating effects of5antioxidants on the liquid preservation of boarsperm and determine the optimal diluents of the antioxidants supplemented in the3selectedextender. The results show that:(1)α-lipoic acid and vitamin C did not show the protective effect on the liquid preservation ofboar sperm in Zorlesco, Mondena and Androhep. They are toxic to the boar sperm preservedin the selected extenders.(2)Taurine is beneficial for the liquid preservation at4℃and17℃. Compared with thecontrol group, groups supplemented with taurine increase boar sperm motility, viability,acrosome integrity, membrane integrity and mitochondrial status significantly, MDA levelwas decreased significantly. The optimal diluent for the3extenders was200μg/mL.(3)The protective effect of vitamin P was verified. During preservation of boar sperm, vitaminP lead to significantly higher boar sperm motility, viability, acrosome integrity, membraneintegrity and mitochondrial status, in comparison with the control groups. The optimal diluentfor liquid preservation of boar sperm was80、120and80μg/mL at4℃, respectively, while 120,80and120μg/mL was optimal for liquid preservation at17℃.(4) L-carnitine show the beneficial effect for the chilled preservation of boar sperm. motility,viability, acrosome integrity, membrane integrity and mitochondrial status was increased forboar sperm stored in the3different extenders, at the same time, MDA level was decreasedsignificantly.120,120and300μg/mL L-carnitine was most beneficial for boar sperm storedin Zorlesco, Androhep and Mondena at4℃, respectively.