The Genetic Diversity Of Captive Forest Musk Deer And The Component Analysis Of Musk

Forest musk deer (Moschus berezovskii) is the level of protection of rare and endangeredwild animals Ⅰ,with high economic value. Farmed deer forest is one of important means toeffectively protect forest musk deer currently wild resources. The face of low level ofmanagement and the lack of scientific breeding forest musk deer farming standards. Show asituation which is confusion gradually pedigree and inbreeding phenomenon highlights.Aimat the present situation currently, it is necessary to conserve genetic diversity,and advocatesto set a standardization production Standard Operating Procedure (SOP) to guide the breedingforest musk deer for Forest musk deer. In terms of genetic diversity, it establish a simple andquick hair DNA extraction methods, with compared to two extraction procedures. Aimed toexplore the feasibility of using hair samples for genetic diversity;This study usedmicrosatellite markers to assay polymorphism of DNA for the breeding population, withUPGMA method to build the phylogenetic tree for forest musk deer populations. The resultswere as follows:1. By comparing the PCR cycler method and boiling method, the result of PCR cyclermethod is higher. With the number of the same hair consistent, PCR cycler method Extractedthe higher concentration of DNA(average concentration for107.7ng/μL). Two methods canbe obtained PCR product, however, the electrophoretic bands of PCR cycler method wereclearer. By PAGE analysis showed that the electrophoretic bands of PCR cycler method wereclearer and effectively meet PAGE detection purposes. Prove the use of PCR cycler methodextracted DNA of animal hairs can serve as a research forest musk deer genetic diversity.2. By using eight microsatellite markers tested and evaluated the genetic diversity ofSixteen forest musk deer in Zhenping county. The results showed that eight microsatellite locicontaining a total of50alleles and average6.25. The observed heterozygosity (Ho) was0.1667~1.0000and the average observed heterozygosity was0.847.The expectedheterozygosity(He) was0.7667~0.8857and the average expected heterozygosity was0.819.PIC polymorphic loci was0.696~0.844. Chi-square test and G statistical tests show that onemicrosatellite loci (Mb41) with Hardy-Weinberg equilibrium, and the remaining sevenmicrosatellite loci were highly significant deviation from H-W equilibrium (P <0.01). 3.With UPGMA method to build the phylogenetic tree for populations analyzed thegenetic relationship between individuals. The result shows that it was the same as consistentwith pedigree of deer farm.4. Through GC-MS component analyzed the forest musk deer in Zhenping county. Theresult shows that it was identified15compounds and the highest was the musk ketone,followed by the3-hydroxy-androstane-17-one and the cholesterol. In addition also containsother steroids and macrocyclic ketones substances.In summary, this paper analyzed the genetic diversity of the forest musk deer breedingpopulation and constructed phylogenetic tree.It improved populations breeding information.The purpose is to provide scientific basis to carry out the development of the post-breedingpopulations pairing scheme and to avoid inbreeding depression. Through analyzing the muskcomposition to providing the basic data of geographical indications certification. The ultimateaim of this study was to provide a theoretical basis which developed a scientific and rationalbreeding genetic resources conservation strategies of the forest musk deer.