Optimized Research On Induction Regenerative Plants From In Vitro Culture Of Unpollinated Ovary And Ovules Of Cucurbita Pepo L.

The effect of different disinfection methods, different slice thickness and different sampling times after treatment of fruit king on embryoid induction were conducted with 8 Pumpkin inbred varieties. Different medium was screened, including the callus bud differentiation medium and rooting medium; at the same time, the regeneration plantlets were acclimated, transplanting method, vitrification seedling prevention and measures to overcome were explored. The results of the study are as follows:1. In vitro culture and plant regeneration of the unpollinated ovary and ovule, disinfecting after section is the best method, The results have showed that its rate of embryos is significantly higher than the treatment of disinfection before section.2. The ovary was treated with fruit setting king when blossoming, the best sampling time of ovule induced embryoid effect is the 14 th day after that.3. The most suitable unfertilized ovary slice thickness of pumpkin is 1.0mm, 1.5mm is next, the worst is 0.5mm, the average rate of embryos are 2.58% 、2.20% and 0.26%.4. The most suitable medium for inducting callus seedling of unfertilized ovary is A4, the formula is MS+20g/LSuc+8g/L agar + 0.5 mg / L NAA+0.5 mg / L 6-BA.5. The time of training shortens to 15 d, and the rate of survival reaches 83.3% with the improved domestication technology B of plantlets.6. The probability of vitrification significantly reduces with cans when culturing, the vitrification are saved using the medium which formula is MS+30g/L Suc+8g/L Agar+0.5mg/L GA3+0.6g/L activated carbon +160mg/L CaCl2 as rooting medium, the effect is especially obvious for minor vitrification plantlets.