| Fatty Liver Hemorrhagic Syndrome(FLHS) is one of the most common nutritional metabolic disease in laying hens(especially laying hens in late period). The sthudy was conducted to investigate prophylactic effects of Polysavone(Alfalfa Extract) on FLHS in Jinghong 1 laying hens which were induced by high-energy low-protein diet.This research was divided into peak period stage and late period stage. The experiment in each stage lasted for 60 days. In peak period, fifty Jinghong 1 laying hens aged 266 days were randomly divided into 5 groups. Hens in group I were fed on a basal diet, while hens in group II were fed on a high-energy low-protein diet and hens in group III, IV, V were fed on the high-energy low-protein diet(the same as group II) supplemented with 200, 600, 1 000 mg/kg ploysavone respectively. The group II was pathological model group and group III, IV, V were revising pathological model groups. In late period, 966 Jinghong 1 laying hens aged 500 days were randomly divided into 7 groups. Hens in group I were fed on a basal diet, while hens in group II were fed on a high-energy low-protein diet and hens in group III, IV, V, VI, VII were fed on the high-energy low-protein diet(the same as group II) supplemented with 200, 400, 600, 800, 1 000 mg/kg ploysavone respectively. The group II was pathological model group and group III, IV, V, VI, VII were revising pathological model groups.The performance, egg quality, the liver items, physiological and biochemical items of plasma lipid metabolism and yolk lipid content of laying hens in each group were measured.Research results as follows:1. Development of model for FLHS in laying hens was induced by high-energy low-protein diet.Development of model for FLHS in laying hens was induced by high-energy low-protein diet(ME 12.85 MJ/kg; CP 13.97%). In peak period, the incidence of FLHS in laying hens was 60% on day 30. In addition, the incidence of FLHS in laying hens was 40% on day 60. In late period, the incidence of FLHS in laying hens was 60% on day 30. In addition, the incidence of FLHS in layin hens was 47% on day 60. Meanwhile, compared with basal diet group, the performance, egg yolk color and yolk lipid content in FLHS pathological model group were significantly different(P<0.05).2. Different dose of Polysavone had prophylactic effects on items of laying hens which were induced FLHS.1) Performance: In peak period, compared with FLHS pathological model group, the ratio of feed to egg in high-energy low-protein diet supplemented with 600 mg/kg ploysavone group was significantly decreased for the first 30 d(P<0.05). In late period, compared with FLHS pathological model group, the ADFI in high-energy low-protein diet supplemented with 600 mg/kg ploysavone group was significantly increased over the 60-day period(P<0.05), while the ratio of feed to egg in high-energy low-protein diet supplemented with 400, 600, 800, 1 000 mg/kg ploysavone group was significantly decreased(P<0.05). Compared with FLHS pathological model group, the laying rate in high-energy low-protein diet supplemented with 400, 600, 800, 1 000 mg/kg ploysavone group was significantly increased over the 60-day period(P<0.05), as well as the egg mass in high-energy low-protein diet supplemented with 200, 400, 600, 800, 1 000 mg/kg ploysavone group(P<0.05).2) Egg Quality: In peak period, compared with FLHS pathological model group, the yolk color was significantly increased in high-energy low-protein diet supplemented with 600 mg/kg ploysavone group on day 42(P<0.05). In late period, compared with FLHS pathological model group, the haugh unit in high-energy low-protein diet supplemented with 200, 400, 600, 800 mg/kg ploysavone group was significantly increased(P<0.05).3) The liver items: In peak period, compared with FLHS pathological model group, the liver hemorrhagic index was significantly decreased in high-energy low-protein diet supplemented with 600, 1 000 mg/kg ploysavone group on day 30(P<0.05), as well as the liver fat content in high-energy low-protein diet supplemented with 200, 600, 1 000 mg/kg ploysavone group(P<0.05). In addition, the incidence of FLHS in high-energy low-protein diet supplemented with 200 mg/kg ploysavone group showed a decrease of 33.3% than high-energy low-protein diet group and no FLHS was found in high-energy low-protein diet supplemented with 600, 1 000 mg/kg ploysavone group on day 30. Moreover, compared with FLHS pathological model group, the incidence of FLHS in high-energy low-protein diet supplemented with 1 000 mg/kg ploysavone group was decreased by 50.0% on day 60. In late period, compared with FLHS pathological model group, the incidence of FLHS in high-energy low-protein diet supplemented with 200, 400, 600, 800, 1 000 mg/kg ploysavone group showed a decrease of 56.7%, 58.3%, 65%, 48.3%, 33.3% on day 30. In addition, compared with FLHS pathological model group, the incidence of FLHS in high-energy low-protein diet supplemented with 200, 400, 600, 800, 1 000 mg/kg ploysavone group showed a decrease of 17%, 42.6%, 48.9%, 53.2%, 36.2% on day 60.4) The physiological and biochemical items of plasma lipid metabolism: Compared with FLHS pathological model group, the items in high-energy low-protein diet supplemented with ploysavone group were not significantly different in peak period and late period(P>0.05).5) Yolk lipid content: In peak period, compared with FLHS pathological model group, the TG in yolk in high-energy low-protein diet supplemented with 200, 600, 1 000 mg/kg ploysavone group was significantly decreased on day 14(P<0.05). And compared with FLHS pathological model group, the total lipid in high-energy low-protein diet supplemented with 1 000 mg/kg ploysavone group was significantly decreased on day 28 and 56(P<0.05), and the TG in yolk in high-energy low-protein diet supplemented with 200, 600, 1 000 mg/kg ploysavone group was significantly decreased(P<0.05), as well as the TG in yolk in high-energy low-protein diet supplemented with 600 mg/kg ploysavone group on day 42(P<0.05). In late period, compared with FLHS pathological model group, the total lipid in high-energy low-protein diet supplemented with 600, 800, 1 000 mg/kg ploysavone group was significantly decreased on day 28(P<0.05), as well as the TG in yolk in high-energy low-protein diet supplemented with 200, 600, 800, 1 000 mg/kg ploysavone group(P<0.05).Conclusion:1. The high-energy low-protein diet(ME 12.85 MJ/kg; CP 13.97%) could induce FLHS in peak period and late period laying hens on day 30.2. Different doses of ploysavone could prevent FLHS in laying hens which were fed high-energy low-protein diet in peak period and late period. In peak period, 600 and 1 000 mg/kg ploysavone could furthest reduce the incidence of FLHS on day 30 and 1 000 mg/kg ploysavone could furthest reduce the incidence of FLHS on day 60. In late period, 600 mg/kg ploysavone could furthest reduce the incidence of FLHS on day 30 and 800 mg/kg ploysavone could furthest reduce the incidence of FLHS on day 60. |
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