Study On Degradation Rate Of Lignin From Different Roughage In Buffalo Rumen

Buffalo can easily raise with low grade forage and the digestibility efficiency of low quality roughage is higher in buffalo than cattle. This study includes 3 hybrid buffalos which are fitted with permanent rumen fistula, using the nylon bag method, electron microscope technology, and in vitro fermentation technology to explore the digestibility of lignin from different feeds in buffalo rumen, and the fermentation characteristics in vitro. The forage resistance of buffalo is revealed by assessing the fiber quality through lignin assessment method. In order to improve the nutrituve value of feed, and increase the breeding benefit provided as a reference for systematic study of the forage resistance mechanism in buffalo by providing a reference feed in buffalo. The results are as follows:(1) The corn stalk, wheat stem, rice stalk, corn stalk silages, rice stalk silage, peanut vine, rice husk, bractea, vinasse and sweet potato vine have a highly degradation rate on different lignin from the listed 10 kinds of feeds, and the effective degradation rate of acid detergent linin(EDADL) big to small are in the order: Peanut vine(15.04%)> Rice straw silage(14.81%)> Corn straw silages(14.78%)> Rice straw(12.05%)> Corn straw(11.97%)> Wheat stem(9.96%)> Bractea(9.78%)> Vinasse(8.38%)> Sweet potato vine(7.22%)> Rice husk(6.29%) respectively. It fully shows the roughage resistance characteristics of buffalo.(2) Comprehensive analysis of these 10 roughages, results showed the acid detergent lignin(ADL) content digestibility of organic matter(DOM) and digestibility of hemicellulose(DHC) showed significantly negative correlation(P<0.05) with digestibility of dry matter(DDM), digestibility of neutral detergent fiber(DNDF), digestibility of acid detergent fiber(DADF) and digestibility of cellulose(DCEL) significantly negative correlations(P<0.01); digestibility of acid detergent lignin(DADL) with DDM, DOM, DCEL and DHC had positively correlated, but the difference is not significant(P > 0.05), and are very significant positive correlation with DNDF(P<0.01), and shows significant positive correlation with DADF(P < 0.05).(3) In vitro fermentation experiments, ADL content in roughage was highly negatively significant correlation with in vitro degradation rate of dry matter(IVDMD)(P < 0.01), and gas production(GP), CO2 and CH4, and the difference was also not significant(P > 0.05).(4) Combined with the experimental results, using ADL and its degradation rate to revise the fermentable fiber index(R-FFI), the fiber quality evaluation results is same in fermentable fiber index(FFI), the fiber quality best for sweet potato vine, the worst was the rice husk; While using digestible neutral detergent fiber(d NDF), relative feed value(RFV) and EDADL to evaluae fiber quality, the results contains certain differences.