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Effencts Of Poplar Seedlings Induced Defense By Tetranychus Urticae Koch On Clostera Anachoreta Fabricus

Posted on:2016-02-15Degree:MasterType:Thesis
Country:ChinaCandidate:C YangFull Text:PDF
GTID:2283330461966335Subject:Forest ecological ecology
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Tetranychus urticae Koch(Acariformes, Tetranychidae) is a worldwide invasive mite. It has a broad range of host plants. Due to the fast propagation and difficulty control, it is becoming a key pest of various crops and trees. Poplar is an important economic wood,commonly heavily damaged by T. urticae. Clostera anachoreta Fabricus(Lepidoptera,Notodontidae) is greedy leaf-chewing insect for poplar. The two types of pests can often be found on the same poplar plant. However, whether there existed a reciprocal influence during their simultaneous infestation on the same host plant is unclear. Therefore, we carried out a study on temporal effect of defense responses of poplars after initial infested by T. urticae and its effects on the feeding behavior, growth and development of C. anachoreta. Some crucial development indices of caterpillars were measured, furthermore a lots of plant physilogical indices including ROS content, related antioxidative enzymes(POD, CAT, APX and AAO)activities,main nutrition substances contents and polyphenol content in poplar leaves infested by T. urticae were determined as well for explanation the potential mechanisms. The aim of this study is to determine the role of defense responses of poplar plants induced by T. urticae in interspecific competition between T. urticae and C. anachoreta and explore its possible mechanism. By investigation the mediation of caterpillars’ growth and development by spider mite infestation on the same poplar seedlings, this research corroborated the effect of induced defensecaused by spider mites which had important theoretical significance for revealing interspecific competition on the same host plant, simultaneously provided new insight for a novel prevention of C. anachoreta. Major results were illustarted as follows:(1) Defense responses of Populus × euramericana had temporal effects induced by T.urticae infestation. At the initial 48 hours infestation by T. urticae, the leaves of poplar sensed the damage from T. urticae at 6h or 12 h, then the antioxidant enzymes(POD, CAT, APX and AAO) activity reached the highest at 12 h, soluble sugar content was lowest at 24 h,polyphenol content was highest at 12 h.(2) Infestation of T. urticae had negative effects on the growth, development and larval feeding behavior of C. anachoreta. The development of C. anachoreta larvae and pupae were significantly restrained, and initial stage’s feeding behavior of larvae was obviously avoided.(3) The potential defense mechanism of poplar plants induced by T. urticae against C.anachoreta were thoroughly discussed. Combined with the temporal effects of induced defense responses of poplar, we found some positive correlation between the defense on C.anachoreta and poplar physiological indices i.e. antioxidant enzymes(POD, CAT, APX, and AAO) activity, polyphenol content and the soluble sugar content, and some negative correlation between the defense on C. anachoreta and soluble sugar content.
Keywords/Search Tags:Tetranychus urticae Koch, Populus × euramericana, Clostera anachoreta Fabricus, induced defense, temporal effect
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