The Basic Study Of Genetic Sexing Strain Of M Elon Fly Bactrocera Cucurbitae (Coquillett)

Melon fly, Bactrocera cucurbitae (Coquillett) is called as the needle bee and Larvae said the melon worm, It has caused serious damage to more than 120 kinds of vegetables and fruits, mainly cucurbitaceae and solanaceae plant, such as melon, pumpkin, cucumber, balsam pear, watermelon, eggplant and tomatoes. It has mainly distributed in Jiangsu, Fujian, Hainan, Guangdong, Guangxi, Huizhou, Yunnan, Sichuan, Hunan, and Taiwan. It is not reported that apply SIT to control melon fly in our country, This study refer to the genetic sexing strain for the oriental fruit fly of the establishment of a genetic sexing strain of melon fly, It is a foundation for use SIT to control melon fly, at the same time, different concentration, different time, Cue-lure effect on the male sexual maturity of melon fly. This study can provide the basis in use sterile insect technique to control melon fly.In order to apply the sterile insect technique (SIT) to control melon fly Bactrocera cucurbitae (Coquillett) in China, the white pupa strain of melon fly was developed. Quality control showed that the egg hatch percent, pupal weight, adult emergence rate, flight ability, and longevity under stress were not significantly different compared with the normal strain. The genetic sexing strain (GSS) of melon fly was developed based on the above white pupa strain. Quality control also showed that the egg hatch percent, pupal weight, adult emergence rate, flight ability, and longevity under stress were not significantly different compared with the normal strain. Genetic recombination rate and phenotype contamination rate tests showed the GSS was stable.Male pupae came from the genetic sexing strain (GSS) of B. cucurbitae were irradiated by 137Cs at the dose of 95,100 and 105Gy, at -1d,-2d and -3d pupal age respectively. The emergence rate, flight ability and survival percentage under stress were tested. The irradiated male adults mated with unirradiated female after emergence, and 100 eggs at 15d,25d and 35d were collected respectively for measuring the hatch rate. The result showed that age of -2d was the optimal pupal age for irradiation and the optimal dose was 100Gy.Sterile males from the irradiated pupae at -2d competed with wild males for wild female to mate. The relative sterile index (RSI) was 0.4605 and the relative Isolation Index (RII) was 1.6346, they meant both sterile male and wild male almost had equal competitiveness.Research about how many days melon fly show the trend to Cue- lure, The result showed that 15 days after the genetic sexing strain (GSS) of melon fly males and 22 days after wild melon fly male would show the trend to Cue- lure. Study on different concentration, different time, Cue- lure effect on the male sexual maturity of melon fly. The experimental data showed that with the increase of time, melon fly male tropism on Cue- lure number also gradually increased, after 1 h, luring effect leveled off. Cue- lure effected on wild melon fly and fertile males and infertile male not exist significant differences.Also can be seen from the picture, under the condition of the same concentration, fertile male temptation effect is more obvious. It showed that irradiation will reduce insect males to the sex lures tropism.