Function Research Of WRKY Transcription Factor Genes From Vitis Davidii 0943 Resistant To White-rot Fungi

White-rot, one of four severe grape diseases, is initiating by Coniothyrium diplodiella. Vitis davidiiis a China wild grape species. Vitis davidii is a kind of wild germplasm resource, belong to East-Asianvitis species, which has a high resistance to grape anthracnose, white rot and colletotrichum. It has beena valuable resource for hot-wet resistance and resistant breeding. Vitis davidii 0943 is a member ofChinese wild brier grape species. It is unisexual and staminiferous plant. This study research thefunction of WRKY transcription factor genes from Vitis davidii 0943 with resistant to white-rot fungi bycontrast with Pinot Noir. The main research results are as follows:1. White-rot fungi was inculated on leaves of Vitis davidii 0943 and Pinot Noir. Vitis davidii 0943 has strong resistance to white-rot fungi by observing the growth of the mycelium.2. According to the known homologous sequences VvWRKY49, VvWRKY53 and Vv WRKY70,primers were designed to clone VdWRKY49, Vd WRKY53 and Vd WRKY70. Through bioinformaticsanalysis of genes and promoters and real-time fluorescence quantitative PCR verified their expressionafter white-rot fungi inoculating and salicylic acid spraying. Then the expression of WRKY49, WRKY53 and WRKY70 were monitored in both Vitis davidii 0943 and Pinot Noir grape which inoculatedwhite rot fungi or salicylic acid by real-time fluorescence quantitative PCR verification. The CDS andpromoter of the Chinese wild grape Vitis davidii 0943 VdWRKY49, VdWRKY53 and VdWRKY70 had theresistance genes feature. At the same time Vd WRKY53 was different to the Pinot Noir grape, the nucleicand animo acid were different. These differences might cause different functions. Bioinformaticsanalysis and real-time fluorescence quantitative PCR indicated that VdWRKY49, Vd WRKY53,VdWRKY70 played important biological function in grape disease resistance. VdWRKY70 showedwhite rot fungus resistant through transgenic functional verification.3. VdWRKY49 did not have a nuclear localization signal and did not locate to the nucleus,VdWRKY53 VdWRKY70 had a nuclear localization signal and located the nucleus by the results ofnucleus localization analysis and expression in Arabidopsis protoplasts positioning.4. Purpose genes were transferred to Arabidopsis by Agrobacterium-mediated. Two Arabidopsispositive strains contained VdWRKY70 were screened. Transgenic Arabidopsis leaves showed resistanceto white rot fungus through inoculation experiments in vitro.