Studies On Ovarian Development And Sperms Transfer In Female Body In Glenea Cantor (Fabricius)

Glenea cantor (Fabricius) is one of the major pests of the kapoke tree, Gossampinus malbarica (DC). The classification of ovarian development, factors influencing ovarian development, and transfer of spermatozoa in G. cantor were examined in laboratory in order to the development of Integrated Pest Management.The main results were summarized as following:1. Rearing method of G. cantor has been improved and made the impact assessment based on previous rearing methods. Results showed that the new method of 50 mL centrifuge tube has some advantages (e. g., simplified rearing procedures, needed little raw material and obtained quantitative population) in comparison of the previous methods. The total survival was not significantly affected by the feed quantity while it was provided above 18.01g. Body weight of adults that as larvae fed on the amount of larval diet from 26.01 to 34.00 g has no significant difference compared with those of natural population.2. The classification of ovarian development of G. cantor can be divided into seven grades. There were 0 (no oocyte), I (early cleavage), II (cleavage stage), III (gravid), IV (ovipositing), V (late egg laying) and VI (postoviposition senescence).3. Nutritorium of kapok has a marked effect on ovarian development in G. cantor. Adult ovary that larvae fed on petiole developed the fastest than those that larvae fed on vein, blade, annotinous and perennial branches.4. Sex ration has obvious effect on ovarian development in G. cantor. The ovarian growth was the fastest with 1 Female:4 Male while it has restrictive effects with sex ratio of 4:1 and 4:0. However, the sex ratio of 1:3, 1:2,1:1,2:1,3:1,1:0,2:0,3:0 has no significant effect on ovarian development.5. Temperature has statistical difference on the ovarian development in G. cantor. Ovarian development gradually accelerated with the increasing of temperature from 15℃ to 30℃. However, ovarian development was restrictive from 30℃ to 35℃.6. During of mating and number of mating has no effect on the ovarian development in G. cantor.7. During of once mating of G. cantor needs for more than 180 min. The phenomenon that sperm was transferred from ejaculatory duct to bursa copulatrix occurs during the mating from the 10 min to 20 min. The sperm was transferred from bursa copulatrix to copulatory pouch occurs during the mating from the 20 min to 40 min. Male completed ejaculation at the 120 min of during of mating. During the process of mating, the amount of spermatozoa in copulatory pouch was gradually increased after the 20min of during of mating, and reach at 4.67 × 104. The amount of spermatozoa in bursa copulatrix increased after the 10 min of during of mating, until up to peak around 120 min. Subsequently, the amount of spermatozoa dropped to 1.68 × 104 at the end of the mating.8. Sperms of G. cantor in copulatory pouch moved to spermatheca at the end of mating. A linear decrease of the number of sperms in copulatory pouch was observed after the second day of mating, and fell to 2.22×104 after the 64 d of mating. However, the number of sperms in spermatheca remained basically unchanged after the end of mating.