The Impact Of The Transgenic Wheat Line With WYMV-NIB8Gene To Physical And Chemical Property, Enzyme Activity And Diversity Of Microbial Community In Rhizsphere

The rhizosphere soil enzymes are the important constituent in soil ecological system. The enzymes mainly come from the microorganism secretion and the root secretion. The enzymes could participate in the important biochemical processes in soil, and they are directly related to the soil fertility. But the transgenic crops may impact the enzyme activity in the rhizosphere soil.At the same time, the rhizosphere microbes are also very important to the soil ecosystems. And the changes of the microorganism ecology in rhizosphere soil could affect the number of the plant pathogenic microorganisms. Ultimately it could lead to the incidence of the serious plant diseases. So detecting the rhizosphere soil microbial community is very important to the rhizosphere ecological security evaluation of the transgenic crops.The transgenic wheat line N12-1with WYMV-Nib8gene which resists wheat yellow mosaic disease and its receptor YM158are from CAAS. The materials were planted in Jiangsu Luhe and Henan Xinxiang in2011and2012. Enzymatic activities of urease, sucrase and dehydrogenase of the rhizospheric soil of N12-1and YM158were analyzed at seeding stage, reviving stage, grain filling stage and mature stage, respectively. Indigo colorimetric method and DNS colorimetric method was respectively used to detecting urease and sucrase. Since the traditional TTC colorimetric method is not suitable for the soil, we established an improved TTC colorimetric method for this test. The physical and chemical properties were tested by the standard method.The microbial diversity of the rhizospheric soil of N12-1and Yangmai158were analysed at seeding stage, seedling stage, reviving stage, heading stage, grain filling stage, mature stage respectively. PCR-DGGE is employed to assess the effect on the bacteria and fungal population diversity.The DGGE fingerprint is analysed by Quantity One. The result of the one way ANOVA shows that there is no significant difference for urease activity, sucrase activity and dehydrogenase activity between N12-1and YM158at both Luhe, Jiangsu Province and Xinxiang, Hernnan Province except dehydrogenase activity at Xinxiang at the mature stage. The enzymatic activity of rhizosphere from different location or different growing stages is different. It indicates that enzyme activity is mainly affected by growing period or soil type.The soil physical and chemical properties are not significant different between N12-1and YM158in terms of one way ANOVA analysis.There is no significant difference between N12-1and YM158at bacteria and fungi community diversity. Based on the principal component analysis (PCA), there are subtle differences between N12-1and YM158in some periods.However there is no significant difference based on the one way ANOVA analysis.