Cockfighting Aggressive Behavior Related Gene5-HT Receptors, MAOA Polymorphism Analysis

Object：Cockfighting is an ancient and rare breeds of poultry germplasm, through along breeding high degree of purification, its offspring have a strong offensive attackand stable performance.For a long time, Turpan cockfighting breeding mainly bytraditional phenotypic selection, molecular genetics and breeding work has not beenreported.Because farming scattered drastically reduced the number of cockfighting,cockfighting included in the national list of animal genetic resources in2006.Thisresearch Turpan cockfighting, the new Roman hens and F1hybrid molecular geneticmarkers in order to find violent aggressive behavior associated marker gene andeffective protection and exploitation cockfighting Turpan.Method：This study uses artificial insemination and natural mating techniques toTurpan cockfighting (♀)×New Roman hens (♀) experiments in batches, three kindof groups performed MAOA and5-HT receptor gene as a candidate gene for PCR-SSCP analysis.Result：（1）The natural mating F1hybrid eggs169, the highest fertility rate detected byincubation with100%minimum57.89%, the highest rate of live embryos was100%Low52.63%. The resulting artificial insemination methods using F1hybrid174eggs,fertilization rate up to100%, the minimum of85.71%, the highest rate of liveembryos was89.47%, minimum75.0%. By X2test fertilization rates significantlydifferent, indicating that artificial insemination can indeed improve fertilization rates.（2） Three groups use PCR-SSCP method analyzed MAOA gene polymorphism in12exon, in the2,3,4,5,10,13exon discovered polymorphic loci. Results show theexon2mutations may be used as cockfighting attacks Turpan selected candidategenes.（3）Three groups use PCR-SSCP method analyzed5-HT receptor genepolymorphism in3exon were analysis of exons in the first and third exon foundpolymorphic loci. Results show the exon1mutation may be used as Turpancockfighting as the aggressive behavior selected candidate genes.Conclusion：Molecular Turpan cockfighting aggressive behavior and MAOA and5-HT receptor gene has a certain relevance, and each one can be chosen as the site ofaggressive behavior in Turpan cockfighting mark, provide molecular evidence forbreeding fighting cocks Turpan stronger attacks.