Selection And Identification Of AT3-11Etc Chinese Cabbage Translocation Lines Introducing Chromosome No.3Segments Of Cabbage

In order to create Chinese cabbage-cabbage translocation lines，irradiation with60Co-γrays and isolated microspore culture were used on Chinese cabbage-cabbage monosomicaddition lines3#(AA+C3). Through cytological and InDel marker analysis on theselfing progenies and microspore culture plants derived from irradiated and un irradiatedplants, eight Chinese cabbage-cabbage translocation lines introduced chromosome3#segments of Cabbage were identified from fifty-four microspore plants and fourty-sixselfing progenies. This study would provide useful materials in genetics and breeding ofChinese cabbage.The main results are shown as follows:1．Isolated microspore culture was performed on Chinese cabbage-cabbage monosomicalien addition lines3#(AA+C3). Through identifying the obtained microspore plants byspecific InDel markers located on Linkage group C02of cabbage, one plant withCabbage specific bands was identified from seventeen microspore plants. Meiosisbehavior of this translocation line showed that the chromosome number is twenty, andwas identified as one Chinese cabbage translocation line introduced one segment ofchromosome3#in Cabbage, naming as AT3-11. By designing multiple InDel markersaround the targeted region, the exogenous Cabbage fragment size is clarified as1.03Mbin this plant. Using one hundred InDel markers uniformly distributed on ten linkagegroups of A genome in Chinese cabbage, the chromosome fragment of Cabbage waspreliminary determined on the chromosome No.5of Chinese cabbage.2．Chinese cabbage-cabbage monosomic alien addition lines3#were irradiated with60Co-γ rays of30Gy,45Gy and60Gy. Then isolated microspore culture was used onthe irradiated plants. Through identification by specific InDel markers from C02Linkagegroups of cabbage on the microspore plants, eight translocation lines with cabbagespecific bands were identified from37microspore plants. Among eight translocationlines, one was derived from plants irradiated with60Co-γ rays of30Gy, five werederived from plants irradiated with60Co-γ rays of45Gy, two were derived from plantsirradiated with60Co-γ rays of60Gy. The results of meiosis behavior of8translocationlines showed that four plants have normal meiosis with20chromosomes, which werepreliminarily identified as translocation lines and were named AT3-24, AT3-45, AT3-47and AT3-54, respectively.3．The pollens of AC3were irradiated with60Co-γ rays of30Gy,45Gy and60Gy. and plants were then selfing to obtain M1seeds. By identification using specific InDelmarkers located on Linkage groups C02of cabbage. Five Chinese cabbage-cabbagetranslocation lines were identified from fourty-six M1plants. The results of meiosisobservation showed that three plants has normal meiosis with20chromosomes, whichwas preliminarily identified as translocation line and were named AT3-4, AT3-23andAT3-39, respectively. Through identification by specific InDel markers on thetwenty-two progenies of AT3-39, seventeen plants had same amplification products withthat of cabbage, some plants had no cabbage specific markers, indicating thattranslocation line AT3-39was heterozygosity.4. Field characters of AT3-11, AT3-45and AT3-54were investigated, and werecompared with that of the controls diploid Chinese cabbage ‘85-1’ and cabbage ‘11-1’.The results showed that there were no clear differences in phenotypic triats at vegetativegrowth stage between AT3-11and one parent Chinese cabbage85-1, while AT3-45andAT3-54had no heading. At the reproductive growth period, AT3-45and AT3-54werelate-bolting and their stigmas were obviously lower than anther, which were similar withcabbage. The results of nutritional quality identification indicated that the Vc content oftranslocation lines was higher than that of the parent Chinese cabbage.