Observation Of Characteristic Of Growth And Development And Molecular Detection Of SRAP Of⁶⁰Co-γ Radio-inducing Mutation For Branches Of Korla Fragrant Pear

This test combined the method of60Co-γ radio-inducing mutation with SRAP molecularmarker aided detection technology,to investigate the method of create new varieties of Korlafragrant pear.The test observing the dormancy branch and sprout branch’s germinationrate,phenological,the growth characteristics of branches, leaves, fruit,comparison the fruitquality and nutrient composition.At the same time, with the help of SRAP molecular markertechnology,from the molecular level to compare the difference between the mutagenic effectsof radiation.The main conclusions are as follows:1.The observation is that, in2013, the bud germination rate of Korla pear radiationprocessing higher than in2012.After the radiation,the pear fruit maturation period in advancefor a week or so than the contrast.40Gy radiation treatment can significantly improve thesprouting branches’ fruit setting rate.Radiation still has inhibitory effect for the branches andleaf’s growth.There is not much difference effect on fruit growth. When the radiation doses inthe range of30-40Gy,the tree body has the stronger adaptability.2.The fruit quality of partial radiation processing branches were improved in variousdegrees.there has a high uniformity fruit shape.Fruit hardness was decreased and commoditywas enhancement.when the dose was20Gy, there were no significant differences among thedormant branches and bud branches in the stone cell content, soluble sugar, soluble solids.The fruit of dormant branches had the highest water content in30Gy. Fruit edible rate andcalyx dropping rate also had improved. In bud branches had the highest stone cellcontent.Germination was significantly higher than that of dormant Branches in fruits’Vc andtitratable acid content. The dose of40Gy sprouting branches had the lowest stone cell content,higher content of soluble sugar and Vitamin C. Its fruit was sweeter. Comprehensivecomparison showed that40Gy sprouting branches fruit has the best quality.3.Due to the different radiation dose and branch types,the contents of nutrient elements isdifferent.The fruit content of Ca、Fe、Mn element was the highest in the sprouting branches of40Gy radiation treatment.The fruit of dormancy branch in30Gy has high content of Cu. Thefruit of dormancy branch in20Gy has high content of Mg.The radiation dose of20-30Gy ishelpful to improve the content of fruit in Zn.4.By SRAP molecular marker assisted detection technology,preliminary determinationthe optimal SRAP-PCR reaction system was20μL.The major is1×PCR Buffer,0.50μmol/Lprimer,0.02mmol/L dNTPs,0.04UTaq DNA polymerase,50ng/μL template DNA,and14.25μL ddH2O.This experiment screened4pairs of primers that are clear, good repeatabilityand high differences and amplified58clear bands in total, polymorphic bands accounted forthe total band’s32.76%.5.Compared the differences between the two types of branches of mutation effect fromthe molecular level.The results showed that with the increase of radiation dose,the differenceof dormancy branch and contrast also increase and in the30Gy radiation dose reached themaximum.When the radiation dose increased again, the difference decreases.The changes ofbudding branches do not show a certain regularity.