Cloning And Expression Of SOD Enzyme After Infection Penicillium And Before Of Hami Melon

Hami melon is a feature of fruits and vegetables in xinjiang, as a result of hami melon in harvestperiod and stored in the process of the easy to rot, so the storage preservation research has always been thehot spot of the research problem,SOD enzyme is a kind of antioxidant enzyme, it can remove free radicals,the present research findings that it closely related to disease resistance and the plant resistance to storage,itis a kind of biological enzyme widely exist in the plant.Through the study of hami melon SOD enzyme hasgreat significance in provides new train of thought and direction to cantaloupe preserve freshness problems.This research is mainly studied the jiashi melon in southern xinjiang with it SOD enzyme activitychanges after the penicillium infect and before,to analysis the SOD part of fragment expression cloning andbioinformatics analysis and the expression of SOD enzyme change in the process of hami melon diseaseafter infection of penicillium and before,To help the preservation of hami melon.(1) Hami melon Cu/zn-SOD enzyme gene cloning and analysis of part of the sequenceUsing PCR technology, cloned Cu/Zn-SOD gene cDNA sequence, named HmCu/ZnSOD, submit theGenebank, it registration number is KF671956.The length cDNA of HmCu/Zn-SOD is403bp, encoding133amino acids, the molecular weight ofamino acid sequence is13649.0kDa, isoelectric point PI value is5.24, The characteristics were analyzedand predicted by the tools of bioinformatics.The results showed that HmCu/Zn-SOD is a hydrophilic,non-transmembrane and non-secretory protein. The main motif of Cu/Zn-SOD in secondary structure wasrandom coils and extended strands. Despite sequence variations, the structure and the composition of aminoacid sequences of HmCu/Zn-SOD were highly similar to that of other green plants.(2) It was studied hami melon Cu/Zn-SOD gene transcription level in different time Using real-timefluorescent quantitative PCR (Real-time PCR, Rt-PCR) methodthe real-time fluorescence quantitative expression analysis indicated that the SOD protein expressionchanged before and after the infection by blue mould. The expression reached the maximum24hours afterthe infection, indicating that the SOD gene plays an important role in the rotting and degeneration of Hamimelons due to the bacterial infection during the preservation.(3) Using chemical method to determined the hami melon SOD enzyme activity change in the processof disease by the method of NBT photoreduction, cantaloupe SOD enzyme activity were determined at differenttime points. Results showed that the SOD activity in different conditions changing with time.the cantaloupe infected SOD enzyme activity in24hours after there will be a peak and then with theextension of time, the decrease of activity. SOD activity among them in the same period, Infected themelon rises above Not infected.