| For Xinjiang soil salinization is increasing, seriously affecting the cotton yield and stableyield, inefficient and excessive use of fertilizers, causing serious environmental pollution andecological deterioration of the environment and other issues. In this study, low-costagricultural products corn flour and soybean meal as raw material, P. putida Rs-198asresearch object, carried out on low-cost media studies. Secondly, bumic acid, urea, and cornflour are as the nutritional support system of PGPR fertilizer. Bentonite, sodium alginate,sodium carboxymethyl cellulose are as additives system of PGPR fertilizer. The nutritionalsupport and additives with the main component chemical fertilizers K2SO4, KCl,(NH4)2HPO4and trace elements boric acid and ammonium molybdate. On the basis of it, they combinedwith PGPR Rs-198. Multifunctional biological compound fertilizer which possesses thefunctions of relieving salt stress, promoting growth, suitable for drip irrigation has beendeveloped. And in laboratory conditions, through drip irrigation methods for investigating themicrobial fertilizer on seed germination rate, seedling rate, biomass accumulation and relatedenzyme activities, verify their biological effects on plants. The results were as follows:The component of low-cost medium was corn flour52.26g/L, soybean meal15.66g/L,K2HPO42.34g/L, MnSO40.18g/L, NaCl5g/L by using response surface optimizationmethod selected ultimately. Strain Rs-198was begins to reach the logarithmic growth phaseat3h in low-cost medium. Strain Rs-198was begins to reach the stability phase at36h. Andstrain growth remained relatively constant within the after60h. The best inoculum of Rs-198was3%, and strain could grow at between20℃to35℃, in which, the best growthtemperature was30℃. The best speed of Rs-198was170rpm, and when100mL low-costmedium was loaded in250mL flask most beneficial the bacteria growth.At room temperature for each nutrient carriers, adjuvants, chemical fertilizers maincomponents, Rs-198strain stability of survival for180days a coating method using thedilution Determination of number of living bacteria, screened and Rs-198good materialcompatibility; screening of each of the above nutrient carriers, additives and chemicalfertilizers main component Rs-198cells with relatively good compatibility, high temperature40-60℃, the water bath15min,30min,45min,60min,90min,120min after thedetermination of the effective number of viable cells, and thus determine the heat resistanceand cell sensitivity to temperature. Four liquid formulation of microbial fertilizer weredetermined tentatively, bacterial suspension (W/W, similarly hereinafter)50%-80%, humicacid1%-4%, urea1%-4%, corn flour4%-7%, bentonite1%-2%, sodium alginate1%-2%,boric acid0.02%-0.04%, ammonium molybdate0.02%-0.2%, K2SO41%-4%, KCl2%-5%,(NH4)2HPO42%-5%, in which, the total nutrients (N+P2O5+K2O) were all over12%. Pot experiment results showed that the germination rate, plant height, root length, freshweight and dry weight under bacterial fertilizer (MF) treatment were increased by35.09~38.86%,10.68%~19.48%,18.13%~26.02%,21.47%~26.44%,7.58%~15.86%thanthat under non-bacterial fertilizer (NMF) treatment in0.3%salt stress condition. Thegermination rate, plant height, root length, fresh weight and dry weight under MF treatmentwere increased by32.91~42.35%,11.84%~21.08%,9.25%~28.57%,24.07%~28.49%,13.85%~21.68%than that under NMF treatment in0.7%salt stress condition. The contents ofmalondialdehyde (MDA) and proline under MF treatment were reduced by32.56%～51.16%and37.52%～87.50%than that under NMF treatment in0.3%salt stress condition. Thecontents of malondialdehyde (MDA) and proline under MF treatment were reduced by22.73%～54.55%and25.03%～66.25%than that under NMF treatment in0.7%salt stresscondition. The superoxide dismutase (SOD), peroxidase (POD), catalase (CAT), polyphenoloxidase (PPO) activities of cotton leaves were effectively reduce under MF treatment thanthat under NMF treatment. |
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