| As the major phosphate storage in crop seeds, phytic acid (PA) usually chelates with K+,Ca2+,Mg2+,Zn2+ions to form phytate. Although PA has a lot of positive physiological functions in plants, human and monogastric animals can not digest phytate directly for on the one hand, its influences to the bioavailability of microelements and the absorption of protein and starch; on the other hand, the excreted phytate polluting the soil and water.,So far, a lot of researches about the influencing factors of the PA content in crop seeds and the relationship between PA and agronomic traits was reported in rice, soybean, corn and other crops, but few PA related reported in cotton. However, studying accumulation characteristics of phytic acid in cottonseed kernels and its relations on main traits of cotton plays an important role in breed improvement of cotton and use ratio increase of cottonseed products, for cottonseed oil and cottonseed meat being one resource of the vegetable oil and animal feed.In this research, Gossypium hirsutum TM-1, Zhe1109, Xiang X121, Zheda31YP and the other7cotton breeds grown in8sites including Hangzhou, Jinhua, Zhoushan and so on were applied as the research subjects, and the ion chromatographic method was optimized and established to measure the PA content in cottonseed kernels. Subsequently, several results about the characteristics of the PA accumulation in cottonseeds and its relationship with the major traits of cottonseeds as well as cotton fiber differentiation and development were preliminarily found and stated as follows:1. Improved extraction condition and optimized sample treatment method was reported in this study by referencing PA measurement methods for other crops and concerning the high oil content of cottonseeds. Also, a quick, accurate ion chromatographic measurement of PA content in cottonseeds was established based on the sample purification by degreasing, shaking and boiling. In this research, R2of regression was0.999suggesting a great linear relationship within the detected concentration. Meanwhile, it was showed that relative standard deviations of all the indexes were smaller than5%, the average recovery rate was98.64%～102.31%, detection limit was0.059μg·mL-1, and the limit of quantitation was0.196μg·mL-1.2. One day after flowering, ovules of Gossypium hirsutum TM-1were choose for in vitro culture by using the medium with different concentrations of PA added. The results showed that exogenous PA could significantly influence the differentiation and development of cotton fiber. The higher the concentration of PA was applied, the stronger inhibition of the differentiation and development of cotton fibers were arised.3. TM-1cottonseeds of20,25,30,35,40days after flowering were taken for the continuous measurement of PA and mineral elements concentration The results showed that PA accumulated fast in the early development stages of the cottonseeds and stabilized35days after flowering. In the cottonseeds, accumulation pattern of P, Fe, Mg, K, Ca and Zn elements was similar to PA except the copper.4.10cotton genotypes from8different planting sites were used for the measurement of cottonseed kernels PA content, cotton fiber quality and cottonseeds physical characteristics. The results showed that the cottonseed kernels PA content was influenced by both genetic and environmental effects, while environmental factors had more significant influence on the variation of PA contents, and phosphate concentration in soil had positive correlation with cottonseed kernels PA content. In the meantime, it was found that cottonseed kernels PA content was positively correlated to ginning outturn, mcronaire and elongation rate, while negatively correlated to the average length of the top half, uniformity, breaking strength, seed index and kernel index. All the correlations were highly significant. |
PDF Full Text Request