| This experiment was conducted to study the effects of adding0.10%lactobacillus delbrueckii to corn-soybean meal diets without antibiotics on growth performance, blood biochemical indexes, fecal cholesterol and bile acid excretion, muscle cholesterol deposition, cholesterol metabolism related enzyme activity and mRNA expression of cholesterol-metabolism related genes. To investigate the mechanism of reducing serum cholesterol by supplying lactobacillus delbrueckii in fatten pigs’diet, and providing scientific basis for the development of farming mode without antibiotics to achieve low cholesterol pork.A total of120healthy crossbred [Duroc×(Landrace×Yorkshire)] finishing pigs with average weight of (65.34±3.64) kg, were randomly divided into2groups, and each group has6stys with10pigs (the number of male and half was equal). The control group was fed basal diet without antibiotics, the experimental group was fed with basal diets containing0.10%lactobacillus delbrueckii. There were7days for pigs to adapt feed before the experiment which lasted for42days. Recording the total feed intake, diarrhea and morbidity rate during the period. At the same time, the weight of pigs in each was recorded respectively at08:00in the morning on the d1and43in the experiment. At08:00in the morning of the d1,28and42, one pig (each group included3male and3female3pigs) was selected from each sty to collect anterior vena cava blood respectively to detect serum biochemical markers, and in the period of d12to14,26to28and40to42d, fresh fecal was collected consecutively for3days to detect fecal cholesterol and cholic acid content.When the experiment was over, one barrow of each sty whose weight was close to the average weight was selected to slaughter after24h limosis, then choosing the same part of ileum, liver, longissimus muscle and myocardium to detect mRNA expression of related genes and enzyme activity of cholesterol metabolism, meanwhile cholesterol content in muscle, was also detected. The results were as follows:1. Supplemented with0.10%Deshi lactobacillus in no antibiotics basal diet can improve ADG, ADFI and decrease F/G of fattening pigs, but there was no significant difference(P>0.05); and compared with the control group, diarrhea rate was extremely significant difference (P<0.01) and morbidity rate was reduced significantly(P<0.05). 2. Compared with control group, on the d14of experiment, serum concent of TBA, TC, TG, LDL-C in text group was decreased, HDL-C and the value of HDL-C/LDL-C was increased, and except TBA there was no significant difference (P>0.05). On d28of experiment there was no significant difference among all the detected serum indexes of2groups (P>0.05), compared with the control group, the content of serum TC, TBA, TG was decreased and LDL-C was increased. On d42of experiment, the content of serum TBA decreased significantly (P＜0.05), and although the content of serum of TC, TG, LDL-C was decreased; HDL-C and the vaule of HDL-C/LDL-C was increased, there was no significant difference (P＞0.05).3. During the period of d12to14, compared with control group, the content of fecal TBA was significantly increased in the experimental group(P<0.05), TC was significantly decreased (P<0.01); d26to28, the content of fecal TBA and TC were higher than the control group, but there the was no significant difference (P＜0.05); d40to42, the content of fecal TBA in text group was significantly higher than the control group (P＜0.05), and TC was lower than control group (P>0.05)4. Compared with control group, the content of TC in liver, myocardium and longissimus muscle was decreased, the content of TBA in liver was decreased, but there was no significant difference (P>0.05).5. Compared with control group, the content of HMGR were decreased (P>0.05), the content of CYP7A1content was increased significantly in liver (P<0.05).6. Compared with control group, the mRNA expression of LDLR and HDLR in liver of test group were increased, and the mRNA expression of ASBT and IBABP in ileum were decreased,but there was no significant difference (P>0.05).The results suggest that adding0.10%lactobacillus delbrueckii in no antibiotics can improve growth performance, reduce cholesterol content in both serum and muscle of fatten pigs.This may be related to lactobacillus delbrueckii in pigs can effects cholesterol and bile acid absorption of intestinal tract, interfere enterohepatic circulation and regulate cholesterol metabolism related enzyme activity in liver. |
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