Genetic Diversity Of Population In Acer Mono Maxim

Acer mono, belonging to Aceraceae, being a perennial deciduous tree, widelydistributed in the Northeast, North of China and the Yangtze River Basin provinces, hasimportant ecological value, medicinal value and economic value. To effectively utilizationand protectation for Acer mono, SRAP and SSR markers were used to reveal the geneticdiversity and genetic structure, investigat genetic differentiation and geographic variation,and explain the relationship between genetic variation and ecological environment, whichwould provide a scientific basis for the effective utilization, development and the protectionof Acer monos.The main results are as follows:1. SRAP results showed that:（1）A total of11pairs of SRAP primer combinationswere selected from88pair primers. Total256bands were amplified, with an average of22.8bands per pair primers. The percentage of polymorphism was99.22%including254polymorphic bands;（2）According to the Shannon diversity index （I）, the highest geneticdiversity was JMLC population（I=0.1450）, while the lowest was FS（I=0.0506）;accordingto the geographical distribution of20populations, the richest genetic diversity was in QinlingMountains, the poorest in Taihang Mountains, the order is:Taihang<Zhongtiao <Lvliang<Yanshan <Taiyue<Qinling；（3） The observed number of alleles （Na）, number ofpolymorphic loci （Np） and the percentage of polymorphic loci （PPB） showed asignificant negative correlation with latitude, other index of genetic diversity were nosignificant or extremely significant correlation with altitude, longitude or latitude;（4） The genetic distance between Yushuwan（YSW）and the Labagou （LBG） was the largest（0.7333）, genetic identity was the smallest （0.4803）; the genetic distance between theBaiyunshan（BYS）and the Qinshui （QS） was the smallest （0.2143）, genetic identitywas the highest （0.8071）;（5） AMOVAshowed that the genetic variation mainly existedamong populations （83%） and only17%within populations;（6） Based on the geneticdistance between populations,20populations mainly clustered into three groups, andbetween the genetic distance and geographic distance was significant correlation.2.SSR results showed that:（1）Atotal of15pairs of SSR primer combinations were selectedfrom20pair primers. Total256bands were amplified, the percentage of polymorphism ashigh as98.44%including254polymorphic bands,（2） According to the Shannon diversityindex （I）, the highest genetic diversity was HBWLS population （I=0.1000）, while thelowest was JMLC （I=0.0181）;according to the geographical distribution of20populations,the richest genetic diversity was in Yanshan mountains, the poorest in Taiyue Mountains. Theorder is: Zhongtiao mountains <Taiyue mountains <Taihang mountains <Lvliang mountains<Qinling mountains<Yanshan mountains;（3） The index of all genetic diversity showed asignificant negative correlation with elevation;and all genetic diversity index were significantpositive correlation with longitude in addition to the effective number of alleles（Ne）;（4）The genetic distance between LBG and QS was the largest （0.6056）. the genetic identitybetween JMLC and HYDXG was the highes（t0.9729）（;5）AMOVAshowed that the geneticvariation mainly existed among populations （89%） and only11%within populations;（6）Based on the genetic distance between populations,20populations mainly clustered intothree groups. while the genetic distance and geographic distance was not significantcorrelation.