Effects Of Addition Recombinant Glucanase And Xylanase To Wheat Based Diets On Production Performance Of Broiler And Layer

In this paper, in order to provide a basis for the application of recombinant xylanase and glucanase in wheat based diets, two experiments were adopted to evaluate effects of addition recombinant glucanase and xylanase to wheat based diets on production performance of broiler and layer.In the first experiment, two hundred and forty Hyline Brown layers were in laying peak were randomly divided into 4 treatment groups, 10 replicates in each treatment. The four treatment groups were corn based diet group(control), experimental group 1(40% wheat substitution), experimental group 2(60% wheat substitution) and experimental group 3(80% wheat substitution). All experimental groups added 5000 U/kg of recombinant β-glucanase originated from Fibrobacter succinogenes and recombinant β-xylanase originated from fungi Neocallimastix frontalis of each. Seven day to adaptation and 28 d for test period. The results showed that compared with the control, wheat based diets supplemented with recombinant glucanase and xylanase, intake, body weight, laying rate, egg weight, and dirty egg rate, egg shape index, eggshell quality, height of egg white, Haugh unit, white weight of yolk and egg had no significant difference(P>0.05), but with increasing substitution ratio, yolk color decreased from 10.67 to 9.47, 7.75 and 6.20 respectively(P<0.05). Broken eggs of experimental groups were significantly lower than that of the control group(P<0.05). As compared with the control, The eggshell thickness of group 1 was thin 0.02mm(P<0.05). The results indicated that addition of recombinant glucanase and xylanase to diets substituted with 40%-80% wheat did not affect the performance and egg quality of Hyline Brown layer during laying peak.In the second experiment, 300 healthy AA broilers with 0 day old, were choiced and randomly divided into 5 groups, with 6 replicates in each, 10 birds per replicate. Feeding experiment lasted 6 weeks. The 5 treatments were: control group 1(corn soybean meal diets, treatment group 1(wheat to replace 40% corn + recombinant enzymes), treatment group 2(wheat to replace 60% corn + enzymes), treatment group 3(wheat to replace 80% corn + enzymes), the control group 2(wheat to replace 60% corn). The addition level of recombinant glucanase and xylanase during 0-21 day old were 10000 U/kg, and 5000 U/kg during 22-42 day age. The results showed that compared with the control group I, body weight, daily gain, feed intake, feed gain ratio, live weight, carcass weight, breast muscle weight, leg muscle weight, gut weight, reducing sugar content in duodenal digesta of treatment groups 1 had no significant difference(P >0.05). At the age of 21 d, the contents of reducing sugar of treatment group 2 and 3 were significantly higher than that in the two control group(P<0.05), and that of treatment group 2 was highest. At the age of 42 d, average daily gain of treatment group 1 was increasd than that of control group by 2.61%, and the weights of duodenum and cecal of treatment group 1 was significantly decreased than that of the control group 1(P<0.05). The results suggest that 40% corn instead by wheat and adding recombinant glucanase and xylanase did not affect the production performance of broilers.