Study On Tissue Culture And Development Of Tube Bulbs In Lycoris Radiata

Lycoris radiata is an economic plant with ornamental, medical and industrial value, widely used in configuration of plant landscape and medicinal production or some other fields. In recent years, the market demand for L. radiata is increasing. Speeding up the cultivation rate of L. radiata to improve production is a clearly issue that needs to be addressed urgently in the industrial production. In this experiment, chosing healthy and strong bulbs, embryos, leaves of L. radiata as explant materials, researehed about every step of the establishment of aseptic system, induction of callus and buds, proliferation and differentiation of callus, enlargement and proliferation of bulblet etc.So as to provide technology support for industrial seedling and expantion of L. radiata, and theoretical basic for tissue culture and rapid propagation of Lycoris. The main results were deseribed as follows:1 The aseptic system was established. Sanitized by 10% NaClO and sieved the better method of disinfection is that cutting the bulb into double scales with base, sterilized 30 seconds by 75% alcohol, then soaked 25 minutes by 10% NaClO with 0.1 ml 2% SDS. Then the Pollution rate was 13.29%, the browning rate was 5.26% and the survive rate was 81.45%.2 Induce the callus and buds of L. radiata. Choosing scales as the suitable explant that has a better effect in inducing callus and buds by comparing the inductive effects of bulbs, leaves and embryos. Then sieved the better scheme of the medium for callus and buds induction of double scales is MS+6-BA 5.0 mg/L+NAA 0.5 mg/L, induction rate of callus was 51.55%, induction rate of buds was 31.07%, total induction rate was 82.62%.3 Proliferation and differentiation of callus. The callus proliferation and adventitious buds differentiation of L. radiata have been researched with method of randomized complete design, using the medium of different hormone proportion. Then sieved the suitable medium for the callus proliferation was MS+6-BA 5.0 mg/L+NAA 0.5 mg/L and its multiplication coefficient was 3.02 after 50-60 d. The suitable medium for the buds differentiation was MS+6-BA 1.0 mg/L+NAA 0.3 mg/L and its differentiation rate was up to 92.58%, and the average number of induced buds was 5.53 after 50-60 d.4 Enlargement and proliferation of bulblet. Influence of the macroelement concentration, sucrose concentration, activated carbon concentration on bulblet enlargement of L.radiata was studied utilizing L9(34) orthogonal design. Then sieved the suitable medium for the bulblet enlargement was MS with 40 g/L sucrose and 1.0 g/L activated carbon. The fresh weight and the diameter of bulblet were up to 860 mg and 1.0 cm respectively. The multiples of the weight gain to 6.62 times. The survival rate was high when transplanted these plants into the cultivation medium with vermiculite. Swollen bulbs of 1.0 cm was cut into 4 pieces and then put into MS+6-BA 5.0 mg/L+NAA 0.5 mg/L for proliferation, the multiplication coefficient was 3.38.