Study On The Optimization Of Fermentation Process Of Cellulose And The Effect Of Acetobacter Pastcurianum To Cattle And Sheep Manure Methane Production

Biogas is a kind of high calorific value, high quality, renewable biomass energy. But the biggest difficulty of biogas engineering technology promotion in the northern area of China biogas fermentation efficiency is low,and the gas production is not stable.Therefore, it has important significance to study the anaerobic biological treatment technology of low temperature conditions in the northern cold area. Have a certain effect on improving gas methane under low temperature environment. This experiment study on this phenomenon that Acetobacter pasteurianus can effectively promote the methane yield increase. The cellulose content of biogas fermentation residue at the same time also increased,to do further study.Through the single factor experiment to optimize the Acetobacter pasteurianus(Acetobacter pasteurianus) ZHC bacteria producing bacterial cellulose fermentation conditions.The experimental results is the best carbon source for glycerol,The best nitrogen source was peptone and yeast extract complex nitrogen source.Optimized Medium for ZHC bacteria is Glycerol 3% Peptone 1% Yeast extract 0.45% disodium hydrogen phosphate 0.2%.30 degrees,Inoculation of 5% ZHC bacteria,Static fermentation for 10 days,Bacterial cellulose production amounted to 4.24 g /L. The analysis results showed that from Biolog ecological plate,The rate of change from the average color(AWCD) could be seen,that Sheep manure50, 51, 53, 60, 64 cow dung, 55, 56, 58,The microbial activity in the eight samples of slurry in the middle of fermentation, were significantly higher than that of fermentation start and end stage.Tust only the microbial activity at the end of fermentation of cow dung 61 65 two kind of slightly above middle.In the middle stage of fermentation is the peak gas production, The Simpson index(D) were relatively low.The Shannon-Wiener index(H ’) value of 15 ℃ higher than 30 ℃.The McIntosh index(U) and the Simpson index(D)all showed the same variation law.Thus the initial description,Under the condition of 15℃ aerobic microbial population abundance is higher than 30 ℃.In the middle stage of fermentation is the peak gas production,The most common microorganisms of the dominance in 15 ℃ and 30 ℃ all showed lower,Species of microbial community evenness were also lower.Principal component analysis showed that the dispersion of sheep manure50, 51, 53, 60, 64 cow dung, 55, 56, 58, between of the eight groups is much more dispersed.To illustrate the corresponding stage of the microbial community metabolic differences carbon is larger.The analysis of DGGE showed that,In 15 degree condition,adding accelerator can effectively improve the advantages abundance reactor bacteria. In different stages of fermentation,Population structure is change, but there are still a total of dominant bacteria.Mainly include Clostridium sp.、Proteiniphilum sp.、Bacteroidales bacterium、Pseudomonas sp etc.Clostridium bacteria showed higher abundances.Fermentation metaphase 30 ℃ DGGE band number was less than 15 ℃,The analysis result is identical with the analysis results of ecological plate.