Candidate Genes And Functional Analysis Of Sexual Prematurity Of Ovaries In Ningdu Yellow Chicken

Ningdu Yellow Chicken is the previous resource for the early seuxal maturity, which meat with high-quality and the characteristic of early sexual maturity. In this study, we prodced a sequence of Tag-DGE of prematurity unproduced ovaries, prematurityproduced ovaries, late-maturing unproduced ovaies and late-maturity produced ovaries.And the qPCR was conduced for the validation of sequence and the relative expression of Guangfeng White Ear yellow chicken’s ovaries. On the base of the aboved results,we screened out the SNPs of gene HEP21 and SOX14 analyzed their correlation analysis between reprduction for further study. The major results were showed as follow:â'ˆ The results of Tag-DGE sequence and enrichment analysis showed that: when P-F-O1 vs P-F-O2, the differential expression genes were 5083, when when L-F-O1 vs L-F-O2, the differential expression genes were 4201. Among the differential expression genes, 1840 genes only appeared in the rematured ovaries and 966 genes only were only appeared in the late-maturity ovaries. The differential expression genes between unproduced and produced ovaries in prematurity chicken gathering to molecular function,the cell position and participation in the biological function were 33, 34 and 52 respectively. They ranked to 69 biochemical pathways and 11 iterms among them were significant enrichment. They were Ribosome, Intestinal immune network for IgA production, Neuroactive ligand-receptor interaction, Herpes simplex infection, Phagosome, Influenza A, Tryptophan metabolism, Cytokine-cytokine receptor interaction, Calcium signaling pathway, NOD-like receptor signaling pathway, Cell adhesion molecules(CAMs). The differential expression genes between unproduced and produced ovaries in late-maturity chicken gathering to molecular function, the cell position and participation in the biological function were 52, 40 and 57 respectively. They ranked to 68 biochemical pathways and 12 iterms among them were significant enrichment. They were Calcium signaling pathway, Intestinal immune network for IgA production, Neuroactive ligand-receptor interaction, Cardiac muscle contraction, Herpes simplex infection, Phagosome, Jak-STAT signaling pathway, Vascular smooth muscle contraction, Dorso-ventral axis formation, Cell adhesion molecules(CAMs),Cytokine-cytokine receptor interaction, ECM-receptor interaction.â'‰ The results of validations of 10 differential expression gene by qPCR showed that the relative expressions 8 genes consistented with the Tag-DGE, which explained the accuracy of DGE. We tested the differential expression between unproduced and produced ovaries of GuangFeng White Ear Chicken of 8 genes(BMP5, BMP15, NHA, INHBA, PTGS1, RRH, HEP21 and SOX14) by qRT-PCR. The expression difference genes of BMP5, INHA, INHBA, RRH and HEP21 were significant., while genes BMP15, PTGS1 and SOX14 were not significant difference.â'Š The SNPs of gene HEP21 and SOX14 were screened out and their correlation analysis were produced between reproduction traits by PCR-RFLP. The result showed that the locis HEP21.A+550G had a certain influence in the 84 days old of crown height(P ﹤0.05), which could be the molecular markers for selection. The locis SOX14.G-1101 A were not mattered with any reproductive performance.By the Tag-DGE of Ningdu Yellow chicken, we could primarily screen out the genes that affected the sexual precocious, which provided a basic molecular basic for the study of native chicken breed in China.