Effects Of Probiotics On Necrotizing Enterocolitis In Neonatal Rats

Necrotizing enterocolitis(NEC) is one of acute gastrointestinal diseases in newborn animals and infants,which is one of the most devastating and unpredictable diseases. The disease mainly affects neonatal, especially premature infants, and with high morbidity and mortality. Despite decades of research, its pathogenesis remains unclear, and it is difficult to diagnose or to treat.The experiment was designed to explore the effects of probiotics on the development of necrotizing enterocolitis in neonatal rat. The animal model of neonatal necrotizing enterocolitis was established in rat and expression change of cytokines IL-6、CXCL1、TNF-α PAF and ITF was detected in the rat intestinal. Methods:Ninety-Six Wistar rats pups were randomly divided into three groups:control group, NEC group and probiotics group. Rats were killed on the fourth day after birth and the terminal of ileum intestines were collected and examined for histopathologic changes. Expression level of IL-6、CXCL1、TNF-α PAF and ITF in the ileum intestine were detected by real-time fluorescent quantitative PCR methods and ELISA methods. Results:Histopathologic score in the NEC group was significantly higher than that in the control group (P<0.01), while histopathologic score in the probiotics group was significantly lower than that in the NEC group (P<0.01). The mRNA relative expression of IL-6 and CXCL1 in the NEC group was significantly higher than that in the control group(P<0.01), and the mRNA relative expression of them in the probiotics group was significantly lower than that in the NEC group (P<0.01), but no significant change in the probiotics group compared to the control group (P>0.05). The mRNA relative expression of TNF-a in the NEC group was higher than that in the control group (P<0.05), while the mRNA relative expression of it in the NEC or control group had no significant change compared to the probiotics group (P>0.05). The mRNA relative expression of PAF in the three experimental groups had no significant different(P>0.05). The mRNA relative expression of ITF in the NEC or probiotics group was significant lower than that in the control group (P<0.01), while it in the probiotics group had no significant change compared to the NEC group (P>0.05). The protein level of IL-6、CXCL1 and TNF-a increased significantly in the NEC group compared to the control group (P<0.01 or P<0.05), while it in the probiotics group was ignificant lower than that in the NEC group (P<0.01 or P<0.05). The protein level of PAF in the NEC or probiotics group was significant lower than that in the control group (P<0.01), while in the probiotics group with no significant change compared to the NEC group (P>0.05). The protein level of ITF in the three experimental groups had no significant different(P>0.05). Conclusion:Probiotics could reduce intestinal inflammatory reaction by regulating the gene and protein expression of IL-6、CXCL1 and TNF-α, but the probiotics had no significant regulation role in the expression of ITF and PAF, the mechanism remains to be further research.