Effects And Mechanisms Of Probiotics Or Probiotics With ND Vaccine On Mucosal Immune Changes Of Chicks

In this experiment, indirect enzymelinked immunosorbent assay(IELlSA), immunohistochemistry staining, histochemistry staining, scanning electron microscope(SEM), transmission electron microscope(TEM) were used to discover the changes and mechanism of the Igs in local humor-tear, trachea fluid, bile, intestinal fluid; the changes and mechanism of both IgS+ antibody-producing cells and T cells in local immune tissues-cecal tonsils, gland of Harder, Peyer's patch of the chicks after administration probiotics or probiotics with ND vaccine. The results were as follows: the content of IgA, IgG, IgM in local humor of probiotics chicks was enhanced compared with those of without probiotics; the numbers of IgA+, IgG+, IgM+ producing cells and T cells in local immune tissues of probiotics chicks were enhanced compared with those of without probiotics; and the length of tiny villus, the numbers of intestinal epithelial lymphocyte(IEL) and lymphoid nodule in cecal tonsils , mitochondrion , rough endoplasmic reticulum, smmooth endoplasmic reticulum , ribosome in lymphocyte were enhanced compared with those of without probiotics. The content of IgA, IgG, IgM in local humor of probiotics chicks vaccinated with ND vaccine was enhanced compared with those of vaccinated with ND which had not been treated with probiotics; the numbers of IgA, IgG, IgM producing cells and T cells in local immune tissues of probiotics chicks vaccinated with ND vaccine were enhanced compared with those of vaccinated with ND vaccine which had not been treated with probiotics were enhanced compared with those of without probiotics. The results showed that humoral and cellular immune function in respiratory path and alimentary tract mucosal were enhanced by probiotics, probiotics could also enhanced the immunopotentiating effects of ND vaccine. After challenged with NDV, the percentage of protection rate of control groupe, probiotics ND groupe, vaccinated groupe were seperatedly: 0%, 100%, 80%, it showed that after probiotics the ablity of vaccinated chicks to resist NDV enhanced. The effects and mechanism of probiotics were illuminated by the changes of Igs content, Igs ' producing cells and T cells numbers in the experiment, which provides scientific guidance for development and application of probiotics which has significance in theory and practice.